Characterization of mouse spermatogonia by transmission electron microscopy
Characterization of mouse spermatogonia by transmission electron microscopy
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DOI：
10.1530/reprod/123.4.567
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发表时间：
2002-04-01
期刊：
影响因子：
3.8
通讯作者：
Russell, LD
中科院分区：
文献类型：
--
作者：
Chiarini-Garcia, H;Russell, LD
用透射电镜观察了C57BL/6J小鼠各种A型、中间型（In）和B型精原细胞的特征。精子原细胞在精子上皮周期的各个阶段被拍照。拍摄了450多张照片。根据核和核仁特征可将精原细胞分为A(s)、A（pr）、A（al）、A(1)、A(2)、A(3)、A(4)、中间型和B型。最原始的精原细胞(A(s)， A（pr)， A(al)）具有斑驳的染色质；A，细胞在整个细胞核中含有均匀的细颗粒染色质；A(2)、A(3)、A(4)和中间型精原细胞包裹核膜的染色质数量逐渐增加；B型精原细胞沿核膜的异染色质较少，但颗粒较中间型精原细胞更致密、更圆。高尔基复合体的线粒体大小和位置在不同类型的精原细胞中有所不同。这些数据表明，精原细胞的类型是可以区分的，因此这些特征可以用于功能研究。
Characteristics of the various type A, intermediate (In) and B spermatogonia were determined in C57BL/6J mice using transmission electron microscopy. Spermatogonia were photographed at all stages of the cycle of the seminiferous epithelium. Over 450 images were taken. Spermatogonia could be classified into A(s), A(pr), A(al), A(1) cells, A(2) cells, A(3) cells, A(4) cells, intermediate type and type B cells primarily on the basis of nuclear and nucleolar characteristics. The most primitive spermatogonia (A(s), A(pr), A(al)) had mottled chromatin; A, cells contained homogeneously finely granular chromatin throughout the nucleus; A(2), A(3), A(4) and intermediate type spermatogonia had progressively increasing amounts of chromatin encrusting the nuclear envelope; type B spermatogonia had less heterochromatin along the nuclear envelope, although the particles were more dense and rounded than in intermediate type spermatogonia. Mitochondrial size and position of Golgi complexes varied in different types of spermatogonia. These data show that types of spermatogonia can be differentiated such that these characteristics can be used in functional studies.