An ortholog of farA of Aspergillus nidulans is implicated in the transcriptional activation of genes involved in fatty acid utilization in the yeast Yarrowia lipolytica.
An ortholog of farA of Aspergillus nidulans is implicated in the transcriptional activation of genes involved in fatty acid utilization in the yeast Yarrowia lipolytica.
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DOI：
10.1016/j.bbrc.2010.10.096
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发表时间：
2010-11
影响因子：
3.1
通讯作者：
Napapol Poopanitpan;Satoshi Kobayashi;R. Fukuda;H. Horiuchi;A. Ohta
中科院分区：
文献类型：
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作者：
Napapol Poopanitpan;Satoshi Kobayashi;R. Fukuda;H. Horiuchi;A. Ohta
酵母菌能有效利用脂肪酸和正构烷烃等疏水底物。为了鉴定解脂耶尔森菌中调节脂肪酸利用的基因(S)，我们首先研究了酿酒酵母OAF1和PIP2的同源基因，但它们的干扰根本没有改变油酸的生长。接下来，我们鉴定了解脂耶尔森菌的一个基因POR1(初级油酸调节因子1)，它是FARA的同源基因，编码一种转录激活因子，调节Nidulans对脂肪酸的利用。POR1缺失突变体对多种脂肪酸的生长有缺陷，但对葡萄糖、甘油和正十六烷无生长缺陷。在正十二烷上有轻微的缺陷。油酸对β氧化和过氧化物体增殖相关基因的转录诱导作用在ΔPpor1菌株中明显减弱。这些数据表明，POR1编码一种转录激活剂，广泛调节解脂耶尔森菌的脂肪酸代谢。
The yeast Yarrowia lipolytica effectively utilizes hydrophobic substrates such as fatty acids and n-alkanes. To identify a gene(s) regulating fatty acid utilization in Y. lipolytica, we first studied homologous genes to OAF1 and PIP2 of Saccharomyces cerevisiae, but their disruption did not change growth on oleic acid at all. We next characterized a Y. lipolytica gene, POR1 (primary oleate regulator 1), an ortholog of farA encoding a transcriptional activator that regulates fatty acid utilization in Aspergillus nidulans. The deletion mutant of POR1 was defective in the growth on various fatty acids, but not on glucose, glycerol, or n-hexadecane. It exhibited slight defect on n-decane. The transcriptional induction of genes involved in β-oxidation and peroxisome proliferation by oleate was distinctly diminished in the Δpor1 strains. These data suggest that POR1 encodes a transcriptional activator widely regulating fatty acid metabolism in Y. lipolytica.