A 28-day oral gavage toxicity study of 3-monochloropropane-1,2-diol (3-MCPD) in CB6F1-non-Tg rasH2 mice.
A 28-day oral gavage toxicity study of 3-monochloropropane-1,2-diol (3-MCPD) in CB6F1-non-Tg rasH2 mice.
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DOI：
10.1016/j.fct.2015.09.019
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发表时间：
2015-12
期刊：
影响因子：
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通讯作者：
Byoung-Seok Lee;Sang-Jin Park;Yongbum Kim;Ji-Seok Han;E. Jeong;Kyoung-Sik Moon;H. Son
中科院分区：
文献类型：
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作者：
Byoung-Seok Lee;Sang-Jin Park;Yongbum Kim;Ji-Seok Han;E. Jeong;Kyoung-Sik Moon;H. Son
3-一氯-1,2-丙二醇（3-MCPD）是一种众所周知的含有水解植物蛋白的食品污染物。然而，可用于3-MCPD风险评估的毒性数据有限，其致癌潜力存在争议。为了评估3-MCPD的潜在毒性并确定剂量水平，对cb6f1 -非Tg rasH2小鼠进行为期26周的致癌性试验，研究人员以0、25、50和100 mg/kg体重(b.w.)/天的剂量，每天1次灌胃给药3-MCPD，连续28天（每剂量雄性和雌性各5只）。标准毒理学评估是在生前和死后阶段进行的。在100 mg/kg体重/天组中，有3名男性和1名女性在研究过程中死亡，并表现出外观消瘦、行为不动、平均体重显著下降等临床症状。显微镜检查显示，100、≥25、≥50、100和100 mg/kg体重/天组肾脏小管嗜碱性粒细胞增多，睾丸精小管管腔退行性生殖细胞脱落，脑空泡化，坐骨神经轴突变性，心肌病。分别。综上所述，3-MCPD的靶器官为肾脏、睾丸、脑、坐骨神经和心脏。3-MCPD的“未观察到不良反应水平”（NOAEL）在男性和女性中分别为≤25和25 mg/kg b.w./day。
3-Monochloro-1,2-propanediol (3-MCPD) is a well-known contaminant of foods containing hydrolyzed vegetable protein. However, limited toxicity data are available for the risk assessment of 3-MCPD and its carcinogenic potential is controversial. To evaluate the potential toxicity and determine the dose levels for a 26-week carcinogenicity test using Tg rasH2 mice, 3-MCPD was administered once daily by oral gavage at doses of 0, 25, 50, and 100 mg/kg body weight (b.w.)/day for 28 days to male and female CB6F1-non-Tg rasH2 mice (N = 5 males and females per dose). The standard toxicological evaluations were conducted during the in-life and post-mortem phase. In the 100 mg/kg b.w./day group, 3 males and 1 female died during the study and showed clinical signs such as thin appearance and subdued behavior accompanied by significant decreases in mean b.w. Microscopy revealed tubular basophilia in the kidneys, exfoliated degenerative germ cells in the lumen of the seminiferous tubule of the testes, vacuolation in the brain, axonal degeneration of the sciatic nerve, and cardiomyopathy in the 100, ≥25, ≥50, 100, and 100 mg/kg b.w./day groups, respectively. In conclusion, 3-MCPD's target organs were the kidneys, testes, brain, sciatic nerve, and heart. The “no-observed-adverse-effect level” (NOAEL) of 3-MCPD was ≤25 and 25 mg/kg b.w./day in males and females, respectively.