The Balance of [Formula: see text] Secretion vs. Reabsorption in the Endometrial Epithelium Regulates Uterine Fluid pH.
The Balance of [Formula: see text] Secretion vs. Reabsorption in the Endometrial Epithelium Regulates Uterine Fluid pH.
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子宫内膜上皮 HCO3 分泌与重吸收的平衡调节子宫液 pH 值
DOI：
10.3389/fphys.2018.00012
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发表时间：
2018
影响因子：
4
通讯作者：
Liu Y
中科院分区：
文献类型：
--
作者：
Xie ZD;Guo YM;Ren MJ;Yang J;Wang SF;Xu TH;Chen LM;Liu Y
子宫液含有高浓度的子宫液，在精子获能和受精过程中起着至关重要的作用。此外，子宫液中的浓度在动情周期期间周期性变化。众所周知，子宫内膜上皮含有涉及顶端 SLC26 家族阴离子交换剂的机制，用于分泌到子宫液中。在本研究中，我们首次发现电中性Na+/协同转运蛋白NBCn1在子宫内膜上皮顶膜表达。顶端 NBCn1 的蛋白质丰度以及顶端 SLC26A4 和 SLC26A6 的蛋白质丰度在子宫动情周期期间相互调节。 NBCn1 在发情间期最丰富，而 SLC26A4/A6 在发情前期/发情期最丰富。在去卵巢小鼠中，子宫NBCn1的表达受到β-雌二醇的抑制，但受到黄体酮的刺激，而子宫SLC26A4/A6的表达受到β-雌二醇的刺激。体内灌注研究表明子宫内膜上皮具有分泌和再吸收能力。此外，子宫内膜上皮的分泌活性在发情期显着高于发情间期。对于重吸收则相反。我们得出的结论是，子宫内膜上皮同时含有涉及顶端 SLC26A4/A6 的分泌活性和涉及顶端 NBCn1 的重吸收活性，并且子宫液中的酸碱稳态是由两种活性的微调平衡调节的。
Uterine fluid contains a high concentration of which plays an essential role in sperm capacitation and fertilization. In addition, the concentration in uterine fluid changes periodically during the estrous cycle. It is well-known that the endometrial epithelium contains machineries involving the apical SLC26 family anion exchangers for secreting into the uterine fluid. In the present study, we find for the first time that the electroneutral Na+/ cotransporter NBCn1 is expressed at the apical membrane of the endometrial epithelium. The protein abundance of the apical NBCn1 and that of the apical SLC26A4 and SLC26A6 are reciprocally regulated during the estrous cycle in the uterus. NBCn1 is most abundant at diestrus, whereas SLC26A4/A6 are most abundant at proestrus/estrus. In the ovariectomized mice, the expression of uterine NBCn1 is inhibited by β-estradiol, but stimulated by progesterone, whereas that of uterine SLC26A4/A6 is stimulated by β-estradiol. In vivo perfusion studies show that the endometrial epithelium is capable of both secreting and reabsorbing . Moreover, the activity for secretion by the endometrial epithelium is significantly higher at estrus than it is at diestrus. The opposite is true for reabsorption. We conclude that the endometrial epithelium simultaneously contains the activity for secretion involving the apical SLC26A4/A6 and the activity for reabsorption involving the apical NBCn1, and that the acid-base homeostasis in the uterine fluid is regulated by the finely-tuned balance of the two activities.
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影响因子：
64.8
作者：
Choi, I;Aalkjaer, C;Boron, WF
通讯作者：
Boron, WF
DOI：
10.1152/ajpregu.00497.2007
发表时间：
2007-12-01
影响因子：
2.8
作者：
Chen, Li-Ming;Choi, Inyeong;Boron, Walter F.
通讯作者：
Boron, Walter F.
影响因子：
15.9
作者：
Lee, MG;Ahn, W;Muallem, S
通讯作者：
Muallem, S
影响因子：
5.5
作者：
Lee, MG;Schultheis, PJ;Muallem, S
通讯作者：
Muallem, S
影响因子：
2.3
作者：
Enuka, Yehoshua;Hanukoglu, Israel;Hanukoglu, Aaron
通讯作者：
Hanukoglu, Aaron