An antimutagenic metabolite, streptovaricin C, isolated from Streptomyces sp.
An antimutagenic metabolite, streptovaricin C, isolated from Streptomyces sp.
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DOI：
10.1248/bpb.22.107
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发表时间：
1999-01
影响因子：
2
通讯作者：
K. Ooka;M. Tanaka;T. Ishikawa;F. Kato
中科院分区：
文献类型：
--
作者：
K. Ooka;M. Tanaka;T. Ishikawa;F. Kato
链霉菌属通过改良Ames试验筛选，从土壤中分离到一株抗诱变剂产生菌KM1-30。经MS和~1H-~(13)C-核磁共振谱分析，该抗突变代谢产物的化学结构为链霉菌素C，它能抑制来自大肠杆菌的DNA依赖的RNA聚合酶和来自RNA肿瘤病毒的依赖于RNA的DNA聚合酶。在经紫外线处理的鼠伤寒沙门氏菌TA100或经Trp-P-2处理的鼠伤寒沙门氏菌TA98的培养物中加入链霉菌素C可降低突变频率，而活细胞数不会减少。链霉菌素C对紫外线和色氨酸-P-2诱变的作用不是去突变，而是抗突变。
Streptomyces sp. KM1-30 was isolated from soil as a producer of antimutagens by screening with a modified Ames test. The chemical structure of the antimutagenic metabolite was identified as streptovaricin C, which is known to inhibit DNA dependent RNA polymerase from E. coli and RNA dependent DNA polymerase from RNA tumor viruses, by MS and 1H-, 13C-NMR analyses. Addition of streptovaricin C to the cultures of UV treated Salmonella typhimurium TA100 or Trp-P-2-treated S. typhimurium TA98 decreased the frequency of mutation without a decrease in viable cell counts. The effect of streptovaricin C to the mutation induced by UV and Trp-P-2 was not desmutagenic, but antimutagenic.