Promoter hypermethylation of the MEG3 (DLK1/MEG3) imprinted gene in multiple myeloma
Promoter hypermethylation of the MEG3 (DLK1/MEG3) imprinted gene in multiple myeloma
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DOI：
10.3816/clm.2008.n.021
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发表时间：
2008-06-01
期刊：
影响因子：
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通讯作者：
Bourantas, Konstantinos L.
中科院分区：
文献类型：
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作者：
Benetatos, Leonidas;Dasoula, Aggeliki;Bourantas, Konstantinos L.
背景资料：甲基化代表了研究最多的表观遗传修饰，并导致参与各种过程（如分化和细胞周期调控）的基因沉默。MEG 3代表在人类中母系表达的印记基因，其编码非翻译产物。本研究对多发性骨髓瘤（MM）中特异性基因的甲基化状态进行了研究。患者和方法：使用甲基化特异性聚合酶链反应（DNA亚硫酸氢盐修饰后）评价了21例MM患者（17例免疫球蛋白[IG] G，3例伊加和1例IgM）。结果：12例（57.14%）骨髓样本和9例（64.28%）外周血样本中发现启动子甲基化。还观察到与疾病阶段和疾病亚型的相关性（IgG，64.7%;伊加，0; IgM，100%）。结论：我们的结论是，在MM患者中观察到MEG 3印迹基因差异甲基化区域的启动子超甲基化。
Background: Methylation represents the most studied epigenetic modification and results in the silencing of genes involved in various processes such as differentiation and cell-cycle regulation. MEG3 represents an imprinted gene maternally expressed in humans that encodes a nontranslated product. In this survey, we studied the methylation status of the specific gene in multiple myeloma (MM). Patients and Methods: Twenty-one patients with MM (17 with immunoglobulin [Ig] G, 3 with IgA, and 1 with IgM) were evaluated using methylation-specific polymerase chain reaction (after DNA bisulphite modification). Results: Promoter hypermethylation was observed in 12 (57.14%) bone marrow samples and in 9 of 14 (64.28%) available peripheral blood samples. A correlation with disease stage was also observed and also with the disease subtype (IgG, 64.7%; IgA, 0; IgM, 100%). Conclusion: We conclude that promoter hypermethylation of the differentially methylated region of the MEG3 imprinted gene is observed in patients with MM.