Green and facile synthesis of iron-doped biomass carbon dots as a dual-signal colorimetric and fluorometric probe for the detection of ascorbic acid
Green and facile synthesis of iron-doped biomass carbon dots as a dual-signal colorimetric and fluorometric probe for the detection of ascorbic acid
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绿色简便地合成铁掺杂生物质碳点作为双信号比色和荧光探针用于检测抗坏血酸
DOI：
10.1039/d1nj05047h
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发表时间：
2021-12-27
影响因子：
3.3
通讯作者：
Xiao, Fubing
中科院分区：
文献类型：
--
作者：
Fan, Pengfei;Liu, Can;Xiao, Fubing
以绿色环保材料奶粉和三氯化铁为原料，采用一步水热法制备了掺铁碳点(Fe-Cd)。结果表明，合成的生物质Fe-Cd具有较强的光致发光性能和较强的模拟氧化酶活性，能够催化3，3‘，5，5’-四甲基联苯胺(TMB)的氧化生成蓝色产物--氧化型TMB(OxTMB)。抗坏血酸(AA)的存在可以还原oxTMB，使溶液颜色变浅，从而实现对AA的比色测定。此外，oxTMB在370 nm处产生的吸收峰与Fe-Cd S的激发光谱重叠，oxTMB通过内滤效应猝灭了Fe-Cd S的荧光。AA的存在会降低oxTMB，恢复Fe-CDS的荧光，且荧光强度的变化与AA的浓度呈正相关。据此，建立了一种新的AA比色/荧光双信号检测方法。比色法的线性范围为25.0~500.0微米，检出限为8.59微米；荧光分析的线性范围为20.0~500.0微米，检出限为5.13微米。该方法利用不同的信号实现互补，消除了单信号的假阳性，提高了检测的准确度，在AA的检测中具有广阔的应用前景。
Iron-doped carbon dots (Fe-CDs) were greenly synthesized via a one-step hydrothermal method using the eco-friendly materials milk powder and FeCl3. It was found that the synthesized biomass Fe-CDs have photoluminescence properties and strong mimetic oxidase activity, which enables catalysis of the oxidation of 3,3 ',5,5 '-tetramethylbenzidine (TMB) to produce a blue product, oxidized TMB (oxTMB). The presence of ascorbic acid (AA) could reduce the oxTMB and make the solution color lighter to realize the colorimetric determination of AA. In addition, the absorption peak of the oxTMB generated at 370 nm overlaps with the excitation spectrum of Fe-CDs, and oxTMB quenched the fluorescence of Fe-CDs based on the internal filtration effect (IFE). The presence of AA will reduce oxTMB and restore the fluorescence of Fe-CDs, and the change of fluorescence intensity is positively correlated with the concentration of AA. Accordingly, a novel method for colorimetric/fluorometric dual-signal detection of AA was developed. The linear range of the colorimetric assay was 25.0-500.0 mu M, and the detection limit was 8.59 mu M. The linear range of the fluorometric assay was 20.0-500.0 mu M with an LOD of 5.13 mu M. The method took advantage of different signals to achieve complementarity and eliminate false positives of single signals and improve the accuracy with great application prospects in the detection of AA.