Characterization of a New Wheat-Aegilops biuncialis 1Mb(1B) Substitution Line with Good Quality-associated HMW Glutenin Subunit
Characterization of a New Wheat-Aegilops biuncialis 1Mb(1B) Substitution Line with Good Quality-associated HMW Glutenin Subunit
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DOI：
10.1556/0806.43.2015.048
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发表时间：
2016-06
影响因子：
1.6
通讯作者：
Jianping Zhou;Yan Cheng;L. Zang;E. Yang;C. Liu;Zheng Xuelian;K. Deng;Y. Zhu;Y. Zhang
中科院分区：
文献类型：
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作者：
Jianping Zhou;Yan Cheng;L. Zang;E. Yang;C. Liu;Zheng Xuelian;K. Deng;Y. Zhu;Y. Zhang
本研究以普通小麦与普通小麦杂交的BC 3F 2后代为材料，选育了一个新的42条染色体代换系12-5-1。CN 19和双刺山羊草（Aegilops buncialis）。12-5-1对白粉病和条锈病均免疫，育性稳定。多色荧光原位杂交结果表明12-5-1为1 Mb（1B）代换系。12-5-1的种子贮藏蛋白电泳结果表明，12-5-1具有CN 19的高分子量麦谷蛋白亚基（2 + 12）和一个新的亚基M，该亚基显然来源于亲本Ae。biuncialis，缺失7 + 8亚基。面粉品质指标表明，12-5-1的蛋白质含量、Zeleny沉淀值、湿面筋含量、籽粒硬度和搅拌时间均显著高于亲本CN 19。此外，5对染色体1 Mb特异性的基于聚合酶链反应的地标独特基因标记，TNAC 1021，TNAC 1026，TNAC 1041，TNAC 1 -02，TNAC 1024，TNAC 1026，TNAC 1044，TNAC 1045，TNAC 1046，TNAC 1047，TNAC 1048，TNAC 1049，TNAC 104
In this study, a new substitution line, 12-5-1, with 42 chromosomes that was derived from BC3F2 descendants of the hybridization between Triticum aestivum cv. CN19 and Aegilops biuncialis was created and reported. The 12-5-1 was immune to both powdery mildew and stripe rust and has stable fertility. Multi-color fluorescence in situ hybridization indicated that 12-5-1 was a substitution line 1Mb(1B). The seed storage protein electrophoresis showed that 12-5-1 presented high molecular weight glutenin subunits (2 + 12) of CN19 and a new subunit designated as M which apparently originated from parent Ae. biuncialis, and absent 7 + 8 subunits. Additionally, the flour quality parameters showed that the protein content, Zeleny sedimentation value, wet gluten content, and grain hardness and mixing time of 12-5-1 were signifiantly higher than those of its parent CN19. Moreover, 5 pairs of the chromosome 1Mb-specifi polymerase chain reaction-based landmark unique gene markers, TNAC1021, TNAC1026, TNAC1041, TNAC1-02...