Nitric oxide regulates the levels of cGMP accumulation in the cricket brain
Nitric oxide regulates the levels of cGMP accumulation in the cricket brain
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DOI：
10.1556/abiol.55.2004.1-4.8
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发表时间：
2004-01-01
影响因子：
--
通讯作者：
Niwa, K
中科院分区：
文献类型：
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作者：
Aonuma, H;Niwa, K
将蟋蟀脑置于含有一氧化氮（NO）供体和磷酸二酯酶抑制剂IBMX的盐水中孵育，IBMX可激活可溶性鸟苷酸环化酶（sGC），使NO靶区cGMP水平升高。NO诱导的cGMP免疫组化显示，许多细胞体的蟋蟀脑cGMP免疫反应性时，制剂处理含有10 mM NO供体SNP和磷酸二酯酶抑制剂IBMX的盐水，但只有少数细胞体显示免疫反应性时，制剂孵育没有NO供体。采用cGMP特异性酶联免疫吸附法测定蟋蟀脑中cGMP的含量。用含有1 μ M NO供体NOR3和1mM IBMX的盐水处理蟋蟀脑。与用含有IBMX的板球盐水处理的对照制剂相比，脑中的cGMP水平增加约75%。当将制剂与含有sGC抑制剂ODQ的NOR3盐水孵育时，cGMP水平降低约40%。这些结果表明，NO激活sGC并增加蟋蟀脑特定神经元中cGMP的水平，并且cGMP的水平将保持在特定水平，这可能调节神经传递中的突触功效。
Cricket brains were incubated in a saline containing nitric oxide (NO)-donor and phosphodiesterase inhibitor IBMX, which could activate soluble guanylate cyclase (sGC) to increase cGMP levels in the targets of NO. The increase of cGMP was detected by immunohistochemistry and enzyme linked immunosorbent assay. NO-induced cGMP immunohistochemistry revealed that many cell bodies of cricket brain showed cGMP immunoreactivity when preparations were treated with a saline containing 10 mM NO-donor SNP and phosphodiesterase inhibitor IBMX, but only a few cell bodies showed immunoreactivity when preparations were incubated without NO-donor. The concentration of cGMP in cricket brains were then measured by using cGMP-specific enzyme linked immunosorbent assay. Cricket brains were treated with a saline containing 1 muM of NO-donor NOR3 and I mM IBMX. The cGMP levels in the brain were increased about 75% compared to control preparations that was treated with a cricket saline containing IBMX The level of cGMP decreased about 40% when preparations were incubated NOR3 saline containing sGC inhibitor ODQ. These results indicate that NO activates sGC and increases the levels of cGMP in particular neurons of the cricket brain and that the level of cGMP would be kept a particular level, which might regulate synaptic efficacy in the neurotransmission.