PPARγ is required for the differentiation of adipose tissue in vivo and in vitro
PPARγ is required for the differentiation of adipose tissue in vivo and in vitro
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DOI：
10.1016/s1097-2765(00)80211-7
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发表时间：
1999-10-01
期刊：
影响因子：
16
通讯作者：
Mortensen, RM
中科院分区：
文献类型：
--
作者：
Rosen, ED;Sarraf, P;Mortensen, RM
脂肪形成的过程涉及多种转录因子的相互作用。已知激活这些因子之一的核激素受体PPAR γ可促进体外脂肪细胞分化。由于缺乏可行的PPAR γ功能丧失模型，因此在体内是否需要PPAR γ参与这一过程仍然是一个悬而未决的问题。我们在这里证明，野生型和PPAR γ零细胞嵌合的小鼠显示零细胞对脂肪组织的贡献很小或没有贡献，而大多数其他器官的正常发育不需要PPAR γ。体外实验表明，胚胎干细胞向脂肪的分化依赖于PPAR γ基因的剂量。这些数据提供了PPAR γ对脂肪形成至关重要的直接证据。
The process of adipogenesis is known to involve the interplay of several transcription factors. Activation of one of these factors, the nuclear hormone receptor PPAR gamma, is known to promote fat cell differentiation in vitro. Whether PPAR gamma is required for this process in vivo has remained an open question because a viable loss-of-function model for PPAR gamma has been lacking. We demonstrate here that mice chimeric for wild-type and PPAR gamma null cells show little or no contribution of null cells to adipose tissue, whereas most other organs examined do not require PPAR gamma for proper development. In vitro, the differentiation of ES cells into fat is shown to be dependent on PPAR gamma gene dosage. These data provide direct evidence that PPAR gamma is essential for the formation of fat.