Identification of Putative Olfactory Genes from the Oriental Fruit Moth Grapholita molesta via an Antennal Transcriptome Analysis.
Identification of Putative Olfactory Genes from the Oriental Fruit Moth Grapholita molesta via an Antennal Transcriptome Analysis.
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通过触角转录组分析鉴定东方果蛾 Grapholitamolesta 的假定嗅觉基因。
DOI：
10.1371/journal.pone.0142193
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Wu J
中科院分区：
文献类型：
--
作者：
Li G;Du J;Li Y;Wu J
东方小食心虫是核果类水果的重要寡食性害虫。作为一种寄主转换物种，成虫，特别是雌蛾，在很大程度上依赖嗅觉线索来定位寄主植物、寻找配偶和选择产卵地点。嗅觉基因的鉴定有助于化学通讯机制的研究。我们使用下一代测序技术生成了G.molesta雌性触角的转录组，并使用Trinity、SOAPdenovo-transs和Abyss-transs组装器组装来自RNA-seq读取的转录物。我们确定了124个假定的嗅觉基因。在已鉴定的嗅觉基因中，118个是该物种的新基因，包括28个编码气味结合蛋白的转录本、17个化学感受蛋白、48个气味受体、4个味觉受体、24个离子型受体、2个感觉神经元膜蛋白和1个气味降解酶。通过半定量逆转录PCR进一步确认了所鉴定的基因，其转录本编码26个OBP和17个CSP。OBP转录本具有明显的触角偏向性，而CSP转录本在不同组织中均有表达。来自东方果蛾的mRNA转录组数据构成了鉴定可能参与该物种嗅觉过程的基因的丰富的分子资源。本研究为进一步研究该害虫的嗅觉识别分子，特别是利用信息素防治该害虫的可行性奠定了基础。
The oriental fruit moth, Grapholita molesta, is an extremely important oligophagous pest species of stone and pome fruits throughout the world. As a host-switching species, adult moths, especially females, depend on olfactory cues to a large extent in locating host plants, finding mates, and selecting oviposition sites. The identification of olfactory genes can facilitate investigation on mechanisms for chemical communications. We generated transcriptome of female antennae of G.molesta using the next-generation sequencing technique, and assembled transcripts from RNA-seq reads using Trinity, SOAPdenovo-trans and Abyss-trans assemblers. We identified 124 putative olfactory genes. Among the identified olfactory genes, 118 were novel to this species, including 28 transcripts encoding for odorant binding proteins, 17 chemosensory proteins, 48 odorant receptors, four gustatory receptors, 24 ionotropic receptors, two sensory neuron membrane proteins, and one odor degrading enzyme. The identified genes were further confirmed through semi-quantitative reverse transcription PCR for transcripts coding for 26 OBPs and 17 CSPs. OBP transcripts showed an obvious antenna bias, whereas CSP transcripts were detected in different tissues. Antennal transcriptome data derived from the oriental fruit moth constituted an abundant molecular resource for the identification of genes potentially involved in the olfaction process of the species. This study provides a foundation for future research on the molecules involved in olfactory recognition of this insect pest, and in particular, the feasibility of using semiochemicals to control this pest.