In vitro assembly of PB2 with a PB1-PA dimer supports a new model of assembly of influenza A virus polymerase subunits into a functional trimeric complex
In vitro assembly of PB2 with a PB1-PA dimer supports a new model of assembly of influenza A virus polymerase subunits into a functional trimeric complex
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DOI：
10.1128/jvi.79.13.8669-8674.2005
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发表时间：
2005-07-01
影响因子：
5.4
通讯作者：
Brownlee, GG
中科院分区：
文献类型：
--
作者：
Deng, T;Sharps, J;Brownlee, GG
流感病毒依赖于RNA的RNA聚合酶是由PB1、PB2和PA组成的异源三聚体复合体。我们发现，单独表达的PB2亚基可以在体外与共表达的PB1-PA二聚体组装成转录活性复合体。此外，我们证明了一个模型病毒RNA启动子可以在与PB2组装之前与PB1-PA二聚体结合。我们的结果与最近提出的病毒RNA聚合酶复合体的顺序组装模型是一致的，在该模型中，PB1-PA二聚体复合体和PB2单体分别被运输到核中，然后在核中组装。
Influenza virus RNA-dependent RNA polymerase is a heterotrimeric complex of PB1, PB2, and PA. We show that the individually expressed PB2 subunit can be assembled with the coexpressed PB1-PA dimer in vitro into a transcriptionally active complex. Furthermore, we demonstrate that a model viral RNA promoter can bind to the PB1-PA dimer prior to assembly with PB2. Our results are consistent with a recently proposed model for the sequential assembly of viral RNA polymerase complex in which the PB1-PA dimeric complex and the PB2 monomer are transported into the nucleus separately and then assembled in the nucleus.