AXONAL-TRANSPORT REVERSAL OF ACETYLCHOLINESTERASE MOLECULAR-FORMS IN TRANSECTED NERVE
AXONAL-TRANSPORT REVERSAL OF ACETYLCHOLINESTERASE MOLECULAR-FORMS IN TRANSECTED NERVE
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DOI：
10.1111/j.1471-4159.1991.tb06403.x
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发表时间：
1991-12-01
影响因子：
4.7
通讯作者：
DIGIAMBERARDINO, L
中科院分区：
文献类型：
--
作者：
HASSIG, R;TAVITIAN, B;DIGIAMBERARDINO, L
本文研究了鸡坐骨神经切断后24小时内，四种分子形式的乙酰胆碱酯酶（AChE）轴突顺行快速转运的抑制。 恢复AChE活性开始约1小时后，神经横断，和所有形式的酶，除了单体的，显示逆转的运输。 酶活性逆转24小时后横断的量是两倍以上，通常通过逆行运输。 我们观察到没有泄漏的酶在该网站的神经横切和AChE活性运输的远端段的切断神经，结果表明，逆行轴突运输所携带的材料不能逆转轴突切断术。 因此，神经横切诱导逆行轴突运输的定量和定性变化，这可能是细胞体远端损伤的信号。 在横断后6小时内测量的反向运输速度为213 mm/天，接近逆行运输速度（200 mm/天）。 这表明在切断的坐骨神经中发生的逆转类似于通常发生在神经末梢处的顺行到逆行转换过程。
Reversal of anterograde rapid axonal transport of four molecular forms of acetylcholinesterase (AChE) was studied in chick sciatic nerve during the 24-h period following a nerve transection. Reversal of AChE activity started approximately 1 h after nerve transection, and all the forms of the enzyme, except the monomeric ones, showed reversal of transport. The quantity of enzyme activity reversed 24 h after transection was twofold greater than that normally conveyed by retrograde transport. We observed no leakage of the enzyme at the site of the nerve transection and no reversal of AChE activity transport in the distal segment of the severed nerve, a result indicating that the material carried by retrograde axonal transport cannot be reversed by axotomy. Thus, a nerve transection induces both quantitative and qualitative changes in the retrograde axonal transport, which could serve as a signal of distal injury to the cell body. The velocity of reverse transport, measured within 6 h after transection, was found to be 213 mm/day, a value close to that of retrograde transport (200 mm/day). This suggests that the reversal taking place in severed sciatic nerve is similar to the anterograde-to-retrograde conversion process normally occurring at the nerve endings.