HUMAN RECOMBINANT INTERLEUKIN-1 STIMULATES COLLAGENASE AND PROSTAGLANDIN E-2 PRODUCTION BY HUMAN SYNOVIAL-CELLS
HUMAN RECOMBINANT INTERLEUKIN-1 STIMULATES COLLAGENASE AND PROSTAGLANDIN E-2 PRODUCTION BY HUMAN SYNOVIAL-CELLS
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DOI：
10.1172/jci112350
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发表时间：
1986-02-01
影响因子：
15.9
通讯作者：
DINARELLO, CA
中科院分区：
文献类型：
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作者：
DAYER, JM;DEROCHEMONTEIX, B;DINARELLO, CA
类风湿关节炎的发病和发展涉及生物活性淋巴因子和单因子的产生。其中，白细胞介素1 （IL-1）一直是一个有争议的分子，因为它似乎在几种不同的组织中引起各种生物反应。在这些研究中，我们证明了人类单核细胞来源的IL-1的三个生物学特性（t淋巴细胞活化和人类滑膜细胞前列腺素E2和胶原酶的产生）共同纯化。我们表达、纯化并检测了人类IL-1突出的pI - 7形式的互补DNA。由于同质重组人IL-1在体外刺激人滑膜细胞的前列腺素E2和胶原酶，并激活T细胞，任何争议现在似乎都解决了。
The pathogegensis and progression of rheumatoid arthritis involves the production of biologically active lymphokines and monokines. Of these, interleukin 1 (IL-1) has been somewhat of a controversial molecule because it seems to evoke various biological responses in several different tissues. In these studies we demonstrate that three biological properties of human monocyte-derived IL-1 (T-lymphocyte activation and human synovial cell prostaglandin E2 and collagenase production) co-purify. The complementary DNA for the prominent pI 7 form of human IL-1 was expressed, purified, and tested. Any controversy now appears resolved since homogeneous recombinant human IL-1 stimulates prostaglandin E2 and collagenase from human synovial cells as well as activates T cells in vitro.