Analysis using a linear viscoelastic model of the in vitro osmotic kinetics of polydisperse synthetic colloids
Analysis using a linear viscoelastic model of the in vitro osmotic kinetics of polydisperse synthetic colloids
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DOI：
10.1021/bm0500143
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发表时间：
2005-05-01
影响因子：
6.2
通讯作者：
Tashiro, C
中科院分区：
文献类型：
--
作者：
Tatara, T;Tashiro, C
这项研究阐明了胶体渗透压 (H) 对整体渗透动力学的贡献以及合成胶体与膜的相互作用。测试了重均分子量 (MWww) 68 800 (DEX 70) 的葡聚糖、MW (40 000 (DEX 40)) 的葡聚糖、MW)(w) (70 000 (HES 70) 的羟乙基淀粉、MW)(w) 60 000 的明胶和白蛋白的溶液 (6%)。使用装有截留分子量大小为 30 000 或 50 000 的膜的渗透流动池来测量每种溶液的 P 值随时间的变化。将线性粘弹性模型拟合到描述 171 作为时间函数的变化的曲线上。 DEX 40 和 DEX 70 的总有效 P 值大于 HES 70、明胶和白蛋白的总有效 P 值。作为膜表面溶质-溶剂交换率的指标，这些值的顺序为DEX 40 > DEX 70、HES 70 > 明胶、白蛋白。研究结果表明，由于初始渗透力较高，DEX 40 可能更适合暂时恢复血浆容量。相反，明胶施加的渗透力增加较慢，但在体内可能持续时间较长，因为明胶与内皮糖萼中带负电的基团相互作用，抑制其穿透毛细血管膜。
This study clarifies the contribution to overall osmotic kinetics of colloid osmotic pressure (H) and the interaction of synthetic colloids with the membrane. Solutions (6%) of dextran with weight average molecular weight (MWww) 68 800 (DEX 70), dextran with MW (40 000 (DEX 40), hydroxyethyl starch with MW)(w) (70 000 (HES 70), gelatin with MW)(w) 60 000 and albumin were tested. An osmotic flow cell fitted with membranes of molecular weight cutoff size 30 000 or 50 000 was used to measure time-dependent changes in P for each of these solutions. A linear viscoelastic model was fitted to the curve describing changes to 171 as a function of time. Values of total effective P for DEX 40 and DEX 70 were larger than those for HES 70, gelatin, and albumin. As an index of solute-solvent exchange rate at the membrane surface, these values were in the order DEX 40 > DEX 70, HES 70 > gelatin, albumin. The findings suggest that DEX 40 may be preferable for the temporary restoration of plasma volume because of a heightened initial osmotic force. In contrast, the osmotic force exerted by gelatin is slower to increase but is likely to be longer lasting in vivo as a result of the inhibition of gelatin from penetrating the capillary membrane due to its interaction with negatively charged groups in the endothelial glycocalyx.