Cardiac tissue engineering: current state and perspectives
Cardiac tissue engineering: current state and perspectives
复制标题
DOI：
10.2741/4002
复制
发表时间：
2012-01-01
影响因子：
3.1
通讯作者：
Radisic, Milica
中科院分区：
文献类型：
--
作者：
Chiu, Loraine L. Y.;Iyer, Rohin K.;Radisic, Milica
心脏组织工程的目标是通过植入工程化的功能性组织替代物或注射细胞和生物材料来治疗心血管疾病，以及提供可用作健康或患病心脏组织的体外模型的工程化心脏结构。随着干细胞技术、材料科学和生物反应器设计的新发现和改进，这一领域正在迅速发展。本文就近年来在心脏组织工程方面取得的一些进展以及未来研究中需要克服的挑战进行综述。主题包括工程干细胞衍生的心脏组织的进展，使用天然或合成聚合物和脱细胞器官作为工程支架，无支架细胞片工程方法，灌注和机械或电刺激在生物反应器中的应用，通过微细加工技术组织心脏细胞，以及体外和体内工程心脏组织的血管化。
The goal of cardiac tissue engineering is to treat cardiovascular diseases through the implantation of engineered functional tissue replacements or the injection of cells and biomaterials, as well as to provide engineered cardiac constructs that can be used as an in vitro model of healthy or diseased heart tissues. This field is rapidly advancing with the new discoveries and improvements in stem cell technologies, materials science, and bioreactor design. In this review, some of the progress made in cardiac tissue engineering in the recent years, as well as the challenges that need to be overcome in future studies, will be discussed. The topics include the advances in engineering stem cell-derived cardiac tissues, the use of natural or synthetic polymers and decellularized organs as engineering scaffolds, the scaffold-free cell sheet engineering approach, the application of perfusion and mechanical or electrical stimulation in bioreactors, the organization of cardiac cells through microfabrication techniques, and the vascularization of engineered cardiac tissues in vitro and in vivo.