Laser capture microdissection as an aid to ultrastructural analysis.
Laser capture microdissection as an aid to ultrastructural analysis.
复制标题
激光捕获显微切割作为超微结构分析的辅助手段。
DOI：
10.1017/s143192760202010x
复制
发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Jerome,WGray
中科院分区：
文献类型：
--
作者：
Grant,Kenneth;Jerome,WGray
Laser capture microdissection uses a microscope to identify specific cells for microdissection and then a laser-sensitive plastic to capture and remove the cells from their substrate. This efficient capture method was originally developed to capture cells for genetic analysis. However, it has also been used to capture cells for proteonomic analysis. In this article, we extend the uses of laser-capture microdissection by reporting a method for preparing captured cells for ultrastructural analysis by transmission electron microscopy. Cells prepared by our methodology show good fine structure preservation and are easily sectioned by standard ultramicrotomy.
