Inhibition of endogenous tissue alkaline phosphatase with the use of alkaline phosphatase conjugates in immunohistochemistry.
Inhibition of endogenous tissue alkaline phosphatase with the use of alkaline phosphatase conjugates in immunohistochemistry.
复制标题
在免疫组织化学中使用碱性磷酸酶缀合物抑制内源性组织碱性磷酸酶。
DOI：
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发表时间：
1981
影响因子：
3.2
通讯作者：
M. M. Wilkinson
中科院分区：
文献类型：
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作者：
B. Ponder;M. M. Wilkinson
在哺乳动物中，碱性磷酸酶有两种形式，一种广泛分布于各种组织中，另一种局限于肠道。左旋咪唑（1-四咪唑）抑制非肠道形式的酶，但对肠道形式没有影响。我们利用犊牛肠碱性磷酸酶偶联物在小鼠组织冷冻切片中进行H2抗原的免疫组织化学证明，利用了这种差异。碱性磷酸酶染色是在1mm左旋咪唑的存在下进行的，左旋咪唑可以抑制内源性组织酶，而不会失去偶联物的染色。内源性酶可以通过其他方式被抑制，例如暴露于20%的乙酸中，但可能会破坏不稳定的抗原。
In mammals there are two forms of alkaline phosphatase, one of which is widely distributed in a variety of tissues, and one of which is confined to intestine. Levamisole (1-tetramisole) inhibits the nonintestinal form of the enzyme, but is without effect on the intestinal form. We have exploited this difference by using conjugates made with calf intestinal alkaline phosphatase for immunohistochemical demonstration of H2 antigens in frozen section of mouse tissues. The alkaline phosphatase staining is performed in the presence of 1 mm levamisole, which inhibits the endogenous tissue enzyme without loss of staining by the conjugate. Endogenous enzyme can be inhibited by other means, such as exposure to 20% acetic acid, but labile antigens may be destroyed.