Altered turnover of hypoxanthine phosphoribosyltransferase in erythroid cells of mice expressing Hprt a and Hprt b alleles.
Altered turnover of hypoxanthine phosphoribosyltransferase in erythroid cells of mice expressing Hprt a and Hprt b alleles.
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表达 Hprt a 和 Hprt b 等位基因的小鼠红细胞中次黄嘌呤磷酸核糖转移酶的周转发生改变。
DOI：
10.1093/genetics/116.2.313
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发表时间：
1987
期刊：
影响因子：
3.3
通讯作者：
Chapman,VM
中科院分区：
文献类型：
--
作者：
Johnson,GG;Chapman,VM
我们先前已经证明，表达Hprt α等位基因的小鼠红细胞次黄嘌呤磷酸核糖基转移酶（HPRT）水平大约是正常小鼠的25倍。（Mus musculus castaneus）和70倍（小鼠spretus）高于表达Hprt球蛋白的小鼠。（小家鼠C57 BI/6 J; C3 H/HeHa），并且红细胞HPRT水平的这些差异是由于网织红细胞成熟为红细胞时HPRT A和B蛋白的转换速率的差异。我们在这里显示：（1）Musus属的分类亚群基本上是单态的，既有Hprt a也有Hprt ballele，Hprt在原住种（M. spretus、Mus hortulanus和Mus abbotti）和几种食蟹动物（Mus musculus musculus、M. M. Castaneus，Mus musculus molossinus），而Hprt常见于野生M. M.（2）在所有的Mus亚群中，Hprt a与红细胞HPRT高水平密切相关，Hprt b与红细胞HPRT低水平密切相关;并且，在本发明中，（3）Hprt α等位基因的出现与红细胞HPRT水平升高之间的相关性在野生型Hprt α等位基因的重复回交后仍然存在遗传背景的近交系小鼠与Hprt球。总的来说，这些观察结果表明红细胞HPRT水平的升高和降低是由Hprt和b结构基因的差异决定的。由于有证据表明Hprt a和b编码的HPRT蛋白质在一级结构上不同，我们推断HPRT的结构是决定其对小鼠红系细胞周转敏感性的重要因素，Hprt a和b可能提供一个有用的“正常”等位基因产物系统，用于鉴定参与小鼠网织红细胞成熟过程中蛋白质周转的因子。
We have previously shown that mice expressingHprt aallele(s) have erythrocyte hypoxanthine phosphoribosyltransferase (HPRT) levels that are approximately 25-fold (Mus musculus castaneus) and 70-fold (Mus spretus) higher than in mice that express theHprt ballele (Mus musculus domesticus; C57BI/6J; C3H/HeHa), and that these differences in erythrocyte HPRT levels are due to differences in the turnover rates of the HPRT A and B proteins as reticulocytes mature to erythrocytes. We show here that: (1) the taxonomic subgroups of the genusMusare essentially monomorphic for the occurrence of either theHprt aor theHprt ballele, withHprt abeing common in the aboriginal species (M. spretus, Mus hortulanusandMus abbotti) and in several commensal species (Mus musculus musculus, M. m. castaneus, Mus musculus molossinus), whileHprt bis common in feralM. m. domesticuspopulations as well as in all inbred strains of mice tested; (2) in all these diverseMussubgroups there is a strict association ofHprt awith high andHprt bwith low levels of erythrocyte HPRT; and, (3) the association between the occurrence of theHprt aallele and elevated erythrocyte HPRT levels is retained following repeated backcrosses of wild-derivedHprt aallele(s) into the genetic background of inbred strains of mice with theHprt ballele. Collectively, these observations indicate that the elevated and low levels of erythrocyte HPRT are specified by differences in theHprt aandbstructural genes. Since evidence indicates thatHprt aandbencode HPRT proteins which differ in primary structure, we infer that the structure of HPRT is an important factor in determining its sensitivity to turnover in mouse erythroid cells.Hprt aandbmay provide a useful system of "normal" allelic gene products for identifying factors that participate in protein turnover during mouse reticulocyte maturation.