Drug treatment of cancer cell lines: a way to select for cancer stem cells?
Drug treatment of cancer cell lines: a way to select for cancer stem cells?
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DOI：
10.3390/cancers3011111
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发表时间：
2011-03-04
期刊：
影响因子：
5.2
通讯作者：
Mondello C
中科院分区：
文献类型：
--
作者：
Chiodi I;Belgiovine C;Donà F;Scovassi AI;Mondello C
肿瘤通常由不同类型的细胞组成。近年来，研究表明，在许多类型的癌症中，有一部分细胞表现出特殊的特征，如移植到宿主动物体内时诱导肿瘤的能力、自我更新和永生的能力，以及产生分化的后代。这些细胞被定义为癌症干细胞（CSCs）或肿瘤起始细胞。基于CSCs能够排除荧光染料、表达特定细胞表面标记物或在特定培养条件下生长的能力，可以从肿瘤标本和已建立的癌细胞系中分离出CSCs。CSCs的一个关键特征是它们对化疗药物的耐药性，这可能有助于治疗后残余癌细胞的残留。研究表明，肿瘤细胞系经药物治疗后可分离出csc样细胞；在这篇综述中，我们将介绍迄今为止用于鉴定和分离csc的策略。此外，我们将讨论这些选择的群体可能用于研究CSC生物学和开发新的抗癌药物。
Tumors are generally composed of different cell types. In recent years, it has been shown that in many types of cancers a subset of cells show peculiar characteristics, such as the ability to induce tumors when engrafted into host animals, self-renew and being immortal, and give rise to a differentiated progeny. These cells have been defined as cancer stem cells (CSCs) or tumor initiating cells. CSCs can be isolated both from tumor specimens and established cancer cell lines on the basis of their ability to exclude fluorescent dyes, express specific cell surface markers or grow in particular culture conditions. A key feature of CSCs is their resistance to chemotherapeutic agents, which could contribute to the remaining of residual cancer cells after therapeutic treatments. It has been shown that CSC-like cells can be isolated after drug treatment of cancer cell lines; in this review, we will describe the strategies so far applied to identify and isolate CSCs. Furthermore, we will discuss the possible use of these selected populations to investigate CSC biology and develop new anticancer drugs.