The ovine cathelicidin SMAP29 kills ovine respiratory pathogens in vitro and in an ovine model of pulmonary infection
The ovine cathelicidin SMAP29 kills ovine respiratory pathogens in vitro and in an ovine model of pulmonary infection
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DOI：
10.1128/aac.45.1.331-334.2001
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发表时间：
2001-01-01
影响因子：
4.9
通讯作者：
Tack, BF
中科院分区：
文献类型：
--
作者：
Brogden, KA;Kalfa, VC;Tack, BF
Cathelicidins是来自绵羊（SMAP29和SMAP34）、兔子（CAP11和CAP18）、啮齿动物（CRAMP）和人类（FALL39、LL37和h/CAP18）的抗菌肽。在肉汤微量稀释试验中，SMAP29、SMAP34、小鼠CRAMP、CAP18、CAP18（31）、CAP18（28）、CAP18（22）和CAP18（21a）活性最强，mic低至0.6 mug/ml。其他的抗菌素活性较低。在患有肺炎的羔羊中，0.5 mg SMAP29降低了支气管肺泡灌洗液和合并肺组织中的细菌浓度。因此，SMAP29的抗菌活性表明它在治疗呼吸道感染方面具有应用价值。
Cathelicidins are antimicrobial peptides from sheep (SMAP29 and SMAP34), rabbits (CAP11 and CAP18), rodents (CRAMP), and humans (FALL39, LL37, and h/CAP18). In a broth microdilution assay against nine ovine pathogens, SMAP29, SMAP34, mouse CRAMP, CAP18, CAP18(31), CAP18(28), CAP18(22), and CAP18(21a) were the most active, with MICs as low as 0.6 mug/ml. Other cathelicidins were less active. In lambs with pneumonia, 0.5 mg of SMAP29 reduced the concentration of bacteria in both bronchoalveolar lavage fluid and consolidated pulmonary tissues. Hence, the antimicrobial activity of SMAP29 suggests that it has applications in the treatment of respiratory tract infections.