Antioxidant properties of different molecular weight polysaccharides from Athyrium multidentatum (Doll.) Ching
Antioxidant properties of different molecular weight polysaccharides from Athyrium multidentatum (Doll.) Ching
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DOI：
10.1016/j.carbpol.2014.03.011
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发表时间：
2014-08-08
影响因子：
11.2
通讯作者：
Sun, Yanlong
中科院分区：
文献类型：
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作者：
Sheng, Jiwen;Sun, Yanlong
采用过氧化氢和抗坏血酸降解从 Athyrium multidentatum (Doll.) Ching 根茎 (CPA) 中提取的多糖，制备不同分子量的多糖。成功获得了四种低分子多糖衍生物（CPA-1、CPA-2、CPA-3和CPA-4），并利用各种已建立的体外系统研究了它们的抗氧化活性。所有CPA衍生物都表现出明显的抗氧化活性，并且在某些测试中比CPA具有更强的抗氧化能力。所有样品中CPA-1对1,1-二苯基1-2-三硝基苯肼(DPPH)自由基的清除能力最强，1050值为25μg/mL。 CPA-2在200μg/mL浓度下对超氧自由基具有最高的清除能力。 CPA-4对羟自由基的清除活性在120～200μg/mL范围内均高于CPA。阐明了CPA及其降解衍生物抗氧化活性的影响机制。 (C) 2014 Elsevier Ltd. 保留所有权利。
Different molecular weight polysaccharides were prepared by degradation of polysaccharides extracted from Athyrium multidentatum (Doll.) Ching rhizome (CPA) with hydrogen peroxide and ascorbic acid. Four low molecular polysaccharides derivatives (CPA-1, CPA-2, CPA-3 and CPA-4) were successfully obtained and had their antioxidant activities investigated employing various established in vitro systems. All CPA derivatives showed pronounced antioxidant activity, and had stronger antioxidant ability than CPA in certain tests. CPA-1 exhibited the strongest scavenging ability on 1,1-dipheny1-2-picrylhydrazyl (DPPH) radical among all samples, and the 1050 value was 25 mu g/mL. CPA-2 possessed the highest scavenging ability against superoxide radical at 200 mu g/mL. The scavenging activity of CPA-4 on hydroxyl radical was higher than CPA from 120 to 200 mu g/mL. The mechanism on influence the antioxidant activity of CPA and its degraded derivatives was indicated. (C) 2014 Elsevier Ltd. All rights reserved.