Partial sciatic nerve ligation induces increase in the phosphorylation of extracellular signal-regulated kinase (ERK) and c-Jun N-terminal kinase (JNK) in astrocytes in the lumbar spinal dorsal horn and the gracile nucleus
Partial sciatic nerve ligation induces increase in the phosphorylation of extracellular signal-regulated kinase (ERK) and c-Jun N-terminal kinase (JNK) in astrocytes in the lumbar spinal dorsal horn and the gracile nucleus
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DOI：
10.1016/s0304-3959(02)00097-0
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发表时间：
2002-09-01
期刊：
影响因子：
7.4
通讯作者：
Quirion, R
中科院分区：
文献类型：
--
作者：
Ma, WY;Quirion, R
炎症和神经损伤引起的脊髓背角和薄核中神经胶质细胞的激活被认为与神经元可塑性和中枢敏化有关，从而导致触觉异常性疼痛。本研究的目的是确定与神经胶质细胞相关的可能的细胞内信号转导途径，该途径已被部分坐骨神经结扎（PSNL）激活，这是一种充分表征的神经性疼痛大鼠模型。损伤后 3 周，PSNL 显着增加了 L4-5 脊髓背角和薄核中的胶质纤维酸性蛋白 (GFAP) 免疫反应性 (IR) 星形胶质细胞。此外，PSNL 增加了这些相同区域的神经胶质细胞中丝裂原激活蛋白 (MAP) 激酶的磷酸化，包括细胞外信号调节激酶 (ERK) 和 c-Jun N 末端激酶 (JNK)，但不包括 p38。磷酸化 (p) ERK- 和 JNK-IR 细胞均与 GFAP 共定位，表明它们在反应性星形胶质细胞中表达。总之，我们的数据表明，PSNL 激活背角和薄核星形胶质细胞中的 ERK/MAP 和 JNK/MAP 激酶途径，这些事件可能与神经性疼痛的发病机制有关。 (C) 2002 年国际疼痛研究协会。由 Elsevier Science B.V. 出版。保留所有权利。
The activation of glial cells in the spinal dorsal horn and the gracile nucleus by inflammation and nerve injury has been suggested to be involved in neuronal plasticity and central sensitization, hence contributing to tactile allodynia. The aim of this study was to determine the possible intracellular signal transduction pathway associated with glial cells, which have been activated by partial sciatic nerve ligation (PSNL), a well-characterized rat model of neuropathic pain. At 3 weeks post-lesion, PSNL markedly increased glia fibrillary acidic protein (GFAP) immunoreactive (IR) astrocytes in both the L4-5 spinal dorsal horn and the gracile nucleus. Moreover, PSNL increased the phosphorylation of mitogen activated protein (MAP) kinases, including the extracellular signal-regulated kinase (ERK) and c-Jun N-terminal kinase (JNK), but not p38, in glia-like cells in these same areas. Both phosphorylated (p) ERK- and JNK-IR cells were co-localized with GFAP, suggesting their expression in reactive astrocytes. In summary, our data indicate that PSNL activates ERK/MAP and JNK/MAP kinase pathways in astrocytes in the dorsal horn and the gracile nucleus, these events possibly being involved in the pathogenesis of neuropathic pain. (C) 2002 International Association for the Study of Pain. Published by Elsevier Science B.V. All rights reserved.