Methods for the Analysis of Polyphosphate in the Life Sciences
Methods for the Analysis of Polyphosphate in the Life Sciences
复制标题
生命科学中多聚磷酸盐的分析方法
DOI：
10.1021/acs.analchem.9b05144
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发表时间：
2020-03-17
影响因子：
7.4
通讯作者：
Blank, Lars Mathias
中科院分区：
文献类型：
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作者：
Christ, Jonas Johannes;Willbold, Sabine;Blank, Lars Mathias
Inorganic polyphosphate (polyP) is the polymer of orthophosphate and can be found in all living organisms. For polyP characterization, one or more of six parameters are of interest: the molecular structure (linear, cyclic, or branched), the concentration, the average chain length, the chain length distribution, the cellular localization, and the cation composition. Here, the merits, limitations, and critical parameters of the state-of-the-art methods for the analysis of the six parameters from the life sciences are discussed. With this contribution, we aim to lower the entry barrier into the analytics of polyP, a molecule with prominent, yet often incompletely understood, contributions to cellular function.
