The first analogues of LNA (Locked Nucleic Acids):: Phosphorothioate-LNA and 2′-thio-LNA
The first analogues of LNA (Locked Nucleic Acids):: Phosphorothioate-LNA and 2′-thio-LNA
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DOI：
10.1016/s0960-894x(98)00366-7
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发表时间：
1998-08-18
影响因子：
2.7
通讯作者：
Wengel, J
中科院分区：
文献类型：
--
作者：
Kumar, R;Singh, SK;Wengel, J
LNA是一种新型的寡核苷酸类似物，能够识别互补的DNA和RNA，具有前所未有的热亲和力。报道了第一个化学修饰的LNA类似物的合成。含有3个胸腺嘧啶单体(1，X=O，Y=S，碱基=胸腺素-1-基)的9-聚硫代硫酸酯-LNA和含有1，3或5个2‘-硫代-LNA单体(1，X=S，Y=O，碱基=尿嘧啶-1-基)的9-聚硫代LNA能够识别互补的DNA和RNA，其热亲和力与亲本LNA相当。(C)1998爱思唯尔科学有限公司。保留所有权利。
LNA (Locked Nucleic Acids, 1, X = O, Y = O) is a novel oligonucleotide analogue capable of recognizing complementary DNA and RNA with unprecedented thermal affinities. Synthesis of the first chemically modified LNA analogues is reported. A 9-mer phosphorothioate- LNA containing three LNA thymine monomers (1, X = O, Y = S, Base = thymin-1-yl) and 9-mer LNAs containing one, three or five 2'-thio-LNA monomers (1, X = S, Y = O, Base = uracil-1-yl) were able to recognize both complementary DNA and RNA with thermal affinities comparable to those of parent LNA. (C) 1998 Elsevier Science Ltd. All rights reserved.