Liquid-Liquid Phase Separation in Oligomeric Peptide Solutions
Liquid-Liquid Phase Separation in Oligomeric Peptide Solutions
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DOI：
10.1021/acs.langmuir.7b01693
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发表时间：
2017-08-08
期刊：
影响因子：
3.9
通讯作者：
Benedek, George B.
中科院分区：
文献类型：
--
作者：
Wang, Ying;Lomakin, Aleksey;Benedek, George B.
寡聚肽广泛存在于生物体中，发挥着广泛的生物学功能。我们报告的第一个观察液液相分离（LLPS）在肽的解决方案，特别是由非共价寡聚体的肽的解决方案。我们确定了寡聚肽溶液的二元相界，并将结果与已建立的球状蛋白质相图进行了比较。我们还提供了简单的理论解释之间的相似性和差异的肽和蛋白质的相图。最后，通过使用拥挤剂调节寡聚体间的相互作用，我们证明了LLPS是一种普遍现象，可以在不同的溶液条件下观察到各种肽。
Oligomeric peptides exist widely in living organisms, and play a role in a broad range of biological functions. We report the first observation of liquid liquid phase separation (LLPS) in peptide solutions, in particular, solutions of peptides consisting of noncovalent oligomers. We determined the binary phase boundary of the oligomeric peptide solution and compared the result to the well-established phase diagram of globular proteins. We also provide simple theoretical interpretations of the similarities and differences between the phase diagrams of peptides and proteins. Finally, by tuning inter-oligomer interactions using a crowding agent, we demonstrated that LLPS is a universal phenomenon that can be observed under different solution conditions for a variety of peptides.