Cytotoxicity of gold nanoparticles with different structures and surface-anchored chiral polymers
Cytotoxicity of gold nanoparticles with different structures and surface-anchored chiral polymers
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不同结构金纳米颗粒和表面锚定手性聚合物的细胞毒性
DOI：
10.1016/j.actbio.2017.01.082
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发表时间：
2017-04-15
影响因子：
9.7
通讯作者：
Gao, Changyou
中科院分区：
文献类型：
--
作者：
Deng, Jun;Yao, Mengyun;Gao, Changyou
纳米颗粒（NPs）对细胞生物学有着深远的影响。然而，具有不同表面手性和结构的金纳米颗粒（AuNPs）的潜在不利影响尚未阐明。在本研究中，将聚（丙烯酰-L（D）-缬氨酸（L（D）-PAV）手性分子单层分别锚定在金纳米立方体（AuNC）和纳米八面体（AuNOs）表面。L-PAV-AuNCs和D-PAV-AuNCs或L-PAV-AuNOs和D-PAV-AuNOs在尺寸、形态和配体密度方面具有相同的物理化学性质，除了颗粒表面上的反向分子手性。L-PAV包被的AuNCs和AuNOs对A549细胞的细胞毒性均大于D-PAV包被的AuNCs，PAV-AuNOs的细胞毒性大于PAV-AuNCs。细胞毒性与细胞摄取量正相关，从而导致细胞内活性氧（ROS）的产生。意义说明制备了不同结构和表面手性的金纳米颗粒，纳米尺度的结构和表面手性可以影响细胞毒性和基因毒性。(C)2017 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
Nanoparticles (NPs) can have profound effects on cell biology. However, the potential adverse effects of gold nanoparticles (AuNPs) with different surface chirality and structures have not been elucidated. In this study, monolayers of poly(acryloyl-L(D)-valine (L(D)-PAV) chiral molecules were anchored on the surfaces of gold nanocubes (AuNCs) and nanooctahedras (AuNOs), respectively. The L-PAV-AuNCs and D-PAV-AuNCs, or the L-PAV-AuNOs and D-PAV-AuNOs, had identical physicochemical properties in terms of size, morphology and ligand density except of the reverse molecular chirality on the particle surfaces, respectively. The L-PAV capped AuNCs and AuNOs exhibited larger cytotoxicity to A549 cells than the D-PAV coated ones, and the PAV-AuNOs had larger cytotoxicity than PAV-AuNCs when being capped with the same type of enantiomers, respectively. The cytotoxicity was positively correlated with the cellular uptake amount, and thereby the production of intracellular reactive oxygen species (ROS).Statement of SignificanceGold nanoparticles with different structure and surface chirality are fabricated.The structure and surface chirality at the nanoscale can influence cytotoxicity and genotoxicity.A new perspective on designing nanoparticles for drug delivery, bioimaging and diagnosis. (C) 2017 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.