Transcriptional and post-transcriptional effects of glucose on liver phosphoenolpyruvate-carboxykinase gene expression.
Transcriptional and post-transcriptional effects of glucose on liver phosphoenolpyruvate-carboxykinase gene expression.
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葡萄糖对肝脏磷酸烯醇丙酮酸羧激酶基因表达的转录和转录后影响。
DOI：
10.1111/j.1432-1033.1991.tb16460.x
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
H. Seitz
中科院分区：
文献类型：
--
作者：
S. Meyer;W. Höppner;H. Seitz
本研究探讨了葡萄糖对肝葡萄糖调节酶磷酸烯醇丙酮酸羧激酶（PPrvck）基因表达的影响。通过使用培养的肝细胞和FAO肝癌细胞，可以证明葡萄糖抑制二丁酰环腺苷酸（Bt 2cAMP），糖皮质激素或两者的作用，增加PPrvck mRNA，从而PPrvck酶活性。葡萄糖具有双重作用;它降低了PPrvck基因的转录，并加速了PPrvck mRNA的降解速率。该效应对葡萄糖具有特异性，因为葡萄糖相关碳水化合物如甘露糖、半乳糖和山梨糖醇对PPrvck mRNA没有影响。葡萄糖的抑制作用仅限于某些蛋白质;葡萄糖对Bt 2 cAMP没有影响，糖皮质激素引起酪氨酸氨基转移酶（达特）的诱导。此外，当FAO肝癌细胞在存在或不存在葡萄糖的情况下孵育时，mRNA体外翻译产物的模式几乎不受影响，证明了葡萄糖对所选蛋白质的基因表达的影响的特异性。在FAO肝癌细胞和培养的肝细胞中，胰岛素和葡萄糖一样，也降低了PPrvck mRNA。虽然葡萄糖和胰岛素的作用是添加剂在FAO肝癌细胞，在原代肝细胞培养的影响，葡萄糖本身对PPrvck mRNA只能证明在胰岛素的情况下。相应地，也在体内，葡萄糖的影响被证明在胰岛素的情况下（引起的链脲佐菌素糖尿病）;葡萄糖的应用减少了肝脏PPrvck mRNA的量。综上所述，葡萄糖能够抑制糖皮质激素和Bt 2cAMP对增加PPrvck mRNA水平的作用。碳水化合物降低PPrvck基因转录速率并加速PPrvck mRNA降解速率。虽然在FAO肝癌细胞中，在胰岛素存在下的效果是明显的，但在培养的肝细胞中，在胰岛素存在下不能证明葡萄糖的效果，质疑其在生理条件下的作用。
The present study investigates the effect of glucose on the gene expression of the hepatic glucoregulatory enzyme, phosphoenolpyruvate carboxykinase (PPrvck). By use of hepatocytes in culture and FAO hepatoma cells it could be demonstrated that glucose suppressed the effect of dibutyryl cyclic AMP (Bt2cAMP), glucocorticoids or both, to increase PPrvck mRNA and consequently PPrvck enzyme activity. Glucose had a dual effect; it reduced PPrvck gene transcription and it accelerated the rate of PPrvck mRNA degradation. The effect was specific for glucose, as glucose-related carbohydrates such as mannose, galactose and sorbitol were without effect on PPrvck mRNA. The repressive effect of glucose was limited to certain proteins; glucose had no effect on Bt2cAMP and glucocorticoid provoked induction of tyrosine aminotransferase (TAT). Also the pattern of mRNA in vitro translation products was virtually unaffected when FAO hepatoma cells were incubated either in the presence or absence of glucose, demonstrating the specificity of the effect of glucose on gene expression of selected proteins. In FAO hepatoma cells and in hepatocytes in culture, insulin, like glucose, also decreased PPrvck mRNA. While the effect of glucose and insulin was additive in FAO hepatoma cells, in primary hepatocytes in culture an effect of glucose by itself on PPrvck mRNA could only be demonstrated in the absence of insulin. Correspondingly also in vivo, the effect of glucose was demonstrated in the absence of insulin (provoked by streptozotocin diabetes); glucose application reduced the amount of hepatic PPrvck mRNA. To summarize, glucose is capable of suppressing the effect of glucocorticoids and Bt2cAMP on increasing the PPrvck mRNA level. The carbohydrate reduces the rate of PPrvck gene transcription and accelerates the rate of PPrvck mRNA degradation. While in FAO hepatoma cells the effect is evident in the presence of insulin, in hepatocytes in culture the effect of glucose cannot be demonstrated in the presence of insulin, questioning its role under physiological conditions.
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DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Wynshaw-Boris,A;Short,JM;Loose,DS;Hanson,RW
通讯作者：
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DOI：
--
发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
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Hod,Y;Hanson,RW
通讯作者：
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DOI：
10.1016/b978-0-12-571142-5.50007-0
发表时间：
1986
期刊：
Recent progress in hormone research
影响因子：
--
作者：
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通讯作者：
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DOI：
--
发表时间：
1990
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
German,MS;Moss,LG;Rutter,WJ
通讯作者：
Rutter,WJ
大鼠肝脏和肾脏中磷酸烯醇丙酮酸羧激酶 mRNA 浓度的快速变化。
DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Cimbala,MA;Lamers,WH;Nelson,K;Monahan,JE;Yoo-Warren,H;Hanson,RW
通讯作者：
Hanson,RW