Immunomodulatory effects of ethanol extract of germinated ice plant (Mesembryanthemum crystallinum).
Immunomodulatory effects of ethanol extract of germinated ice plant (Mesembryanthemum crystallinum).
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DOI：
10.5625/lar.2017.33.1.32
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发表时间：
2017-03-01
影响因子：
2.9
通讯作者：
Park, Jong-Hwan
中科院分区：
文献类型：
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作者：
Choi, Joo-Hee;Jo, Sung-Gang;Park, Jong-Hwan
研究冰草提取物（IPE）的免疫调节活性。用各种剂量的IPE处理Raji（人B细胞系）和Jurkat（人T细胞系）细胞，并通过WST测定法测量细胞增殖。结果表明，IPE能促进Raji和Jurkat细胞的增殖，并呈剂量依赖性。IPE还增强了脂多糖（LPS）存在下巨噬细胞中IL-6和TNF-α的产生，尽管IPE单独不诱导细胞因子的产生。此外，IPE治疗上调iNOS基因的表达在巨噬细胞中的时间和剂量依赖性的方式，并导致产生一氧化氮在巨噬细胞中的IFN γ的存在。体内研究表明，口服IPE 2周可增加脾细胞中CD 4+、CD 8+和CD 19+细胞的分化。这些结果表明，IPE具有免疫调节作用，可以开发为免疫调节补充剂。
The purpose of this study was to investigate the immunomodulatory activity of ice plant (Mesembryanthemum crystallinum) extract (IPE) in vitro and in vivo. Raji (a human B cell line) and Jurkat (a human T cell line) cells were treated with various doses of IPE and cell proliferation was measured by WST assay. Results showed that IPE promoted the proliferation of both Raji and Jurkat cells in a dose-dependent manner. IPE also enhanced IL-6 and TNF-alpha production in macrophages in the presence of lipopolysaccharide (LPS), although IPE alone did not induce cytokine production. Moreover, IPE treatment upregulated iNOS gene expression in macrophages in a time- and dose-dependent manner and led to the production of nitric oxide in macrophages in the presence of IFNgamma. In vivo studies revealed that oral administration of IPE for 2 weeks increased the differentiation of CD4+, CD8+, and CD19+ cells in splenocytes. These findings suggested that IPE has immunomodulatory effects and could be developed as an immunomodulatory supplement.