Accelerated bonelike apatite growth on porous polymer/ceramic composite scaffolds in vitro
Accelerated bonelike apatite growth on porous polymer/ceramic composite scaffolds in vitro
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DOI：
10.1089/ten.2006.12.2997
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发表时间：
2006-10-01
影响因子：
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通讯作者：
Kim, Byung-Soo
中科院分区：
文献类型：
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作者：
Kim, Sang-Soo;Park, Min Sun;Kim, Byung-Soo
虽然生物可降解聚合物/陶瓷复合支架可以克服传统陶瓷骨替代物的局限性，这些支架的成骨潜力需要进一步提高，以有效的骨组织工程。在这项研究中，类骨磷灰石有效地涂覆到支架表面，通过使用聚合物/陶瓷复合支架，而不是聚合物支架，并通过使用加速仿生过程，以提高支架的成骨潜力。通过在模拟体液（SBF）中孵育支架来实现骨样磷灰石涂层聚合物支架的创建。多孔聚乳酸羟基乙酸/纳米羟基磷灰石（PLGA/HA）复合支架上磷灰石的生长速度明显快于多孔PLGA支架。此外，涂层磷灰石在PLGA/HA支架上的分布比在PLGA支架上更均匀。在5天的孵育期后，在5 x SBF中孵育的PLGA/HA支架上涂覆的磷灰石的质量比在1 x SBF中孵育的PLGA/HA支架高2.3倍。此外，当支架在5x SBF中孵育5天时，涂覆在PLGA/HA支架上的磷灰石的质量比PLGA支架高4.5倍。这些结果表明，可以通过使用聚合物/陶瓷复合支架和浓缩的SBF来加速仿生磷灰石涂层。当接种成骨细胞，磷灰石涂层的PLGA/HA支架表现出显着更高的细胞生长，碱性磷酸酶活性，和矿化在体外相比，磷灰石涂层的PLGA支架。因此，磷灰石涂层的PLGA/HA支架材料作为骨组织工程支架材料时，可提供增强的成骨潜能。
Although biodegradable polymer/ceramic composite scaffolds can overcome the limitations of conventional ceramic bone substitutes, the osteogenic potential of these scaffolds needs to be further enhanced for efficient bone tissue engineering. In this study, bonelike apatite was efficiently coated onto the scaffold surface by using polymer/ceramic composite scaffolds instead of polymer scaffolds and by using an accelerated biomimetic process to enhance the osteogenic potential of the scaffold. The creation of bonelike, apatite-coated polymer scaffold was achieved by incubating the scaffolds in simulated body fluid (SBF). The apatite growth on porous poly(D,L-lactic-co-glycolic acid)/nanohydroxyapatite (PLGA/HA) composite scaffolds was significantly faster than on porous PLGA scaffolds. In addition, the distribution of coated apatite was more uniform on PLGA/HA scaffolds than on PLGA scaffolds. After a 5-day incubation period, the mass of apatite coated onto PLGA/HA scaffolds incubated in 5 x SBF was 2.3-fold higher than PLGA/HA scaffolds incubated in 1 x SBF. Furthermore, when the scaffolds were incubated in 5 x SBF for 5 days, the mass of apatite coated onto PLGA/HA scaffolds was 4.5-fold higher than PLGA scaffolds. These results indicate that the biomimetic apatite coating can be accelerated by using a polymer/ceramic composite scaffold and concentrated SBF. When seeded with osteoblasts, the apatite-coated PLGA/HA scaffolds exhibited significantly higher cell growth, alkaline phosphatase activity, and mineralization in vitro compared to the apatite-coated PLGA scaffolds. Therefore, the apatite-coated PLGA/HA scaffolds may provide enhanced osteogenic potential when used as scaffold for bone tissue engineering.