Photoactivation of toxin conjugates.
Photoactivation of toxin conjugates.
复制标题
毒素结合物的光活化。
作者：
V. Goldmacher;P. Senter;J. Lambert;W. Blättler
A novel photocleavable protein cross-linking reagent has been used for conjugation of the ribosome-inactivating protein pokeweed antiviral protein from seeds of Phytolacca americana (PAP-S), with either the monoclonal antibody 5E9 directed against the human transferrin-receptor or the B-chain of ricin that binds to cell-surface oligosaccharides bearing terminal D-galactose residues. When irradiated with near-UV light (350 nm), the linker of these conjugates undergoes photolytic degradation, resulting in the release of native toxin that is fully functional. The cytotoxicities of these 5E9-PAP-S and ricin B-chain-PAP-S conjugates for HeLa cells could be enhanced by irradiating the cells with light after they had internalized the conjugates.
定义细胞活化的人细胞表面抗原的单克隆抗体 (5E9) 的表征。
DOI：
--
发表时间：
1981
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Haynes,BF;Hemler,M;Cotner,T;Mann,DL;Eisenbarth,GS;Strominger,JL;Fauci,AS
通讯作者：
Fauci,AS
DOI：
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发表时间：
1990-11
期刊：
The Journal of biological chemistry
影响因子：
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作者：
E. Feener;Wei-Chiang Shen;Hugues J.-P. Ryser
通讯作者：
E. Feener;Wei-Chiang Shen;Hugues J.-P. Ryser