Pure culture and cytological properties of ‘Syntriphobacter wolini’
Pure culture and cytological properties of ‘Syntriphobacter wolini’
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‘Syntriphobacter wolini’的纯培养物和细胞学特性
DOI：
10.1111/j.1574-6968.1994.tb07232.x
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发表时间：
1994
影响因子：
2.1
通讯作者：
B. Schink
中科院分区：
文献类型：
--
作者：
Christina Wallrabenstein;E. Hauschild;B. Schink
互养丙酸盐氧化细菌沃氏互养菌在二元产甲烷共培养物中与亨氏甲烷菌作为唯一伴侣生长，不含污染的脱硫弧菌。底物试验表明，S. Wolinii也可以在没有任何互养伙伴的情况下生长，无论是以丙酮酸为唯一底物还是以丙酸盐加硫酸盐。丙酮酸发酵成乙酸盐、丙酸盐和可能的CO2;丙酸盐在硫酸盐存在下不完全氧化成乙酸盐和CO2，形成化学计量的硫化物。纯培养物含有细胞色素B和c甲基萘醌-7。未检测到脱硫韦啶或硫代韦啶。
The syntrophically propionate-oxidizing bacterium Syntrophobacter wolinii was grown in binary methanogenic coculture with Methanospirillum hungatei as sole partner, free of contaminating Desulfovibrio vulgaris. Substrate tests revealed that S. wolinii could grow also without any syntrophic partner, either with pyruvate as sole substrate or with propionate plus sulfate. Pyruvate was fermented to acetate, propionate and presumably CO2; propionate in the presence of sulfate was oxidized incompletely to acetate and CO2, with stoichiometric sulfide formation. The pure culture contained cytochromes b and c menaquinone-7. Desulfovirdin or desulforubidin could not be detected.