Characterization of microRNA expression profiles in normal human tissues.
Characterization of microRNA expression profiles in normal human tissues.
复制标题
正常人组织中microRNA表达谱的表征。
DOI：
10.1186/1471-2164-8-166
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发表时间：
2007-06-12
期刊：
影响因子：
4.4
通讯作者：
Chen, Caifu
中科院分区：
文献类型：
--
作者：
Liang, Yu;Ridzon, Dana;Wong, Linda;Chen, Caifu
测定不同生理和病理条件下组织中miRNAs的含量是研究miRNAs功能的重要第一步。正常状态下的匹配样本可以为分析miRNA丰度的变化提供必要的基线参考。我们提供了345个miRNAs在40个正常人体组织中的表达数据，这些数据确定了普遍表达的miRNAs，以及几组仅在某些组织类型中表达或优先表达的miRNAs。许多具有共调节表达模式的miRNA位于相同的基因组簇内，并且可以通过比较基因组策略来鉴定控制其表达模式的候选转录因子。正常组织通过其miRNA表达谱的层次聚类基本上遵循器官的结构、解剖位置和生理功能，这表明可以通过与其独特表达的组织的生物学相联系来理解miRNA的功能。基于数据库检索，发现许多在脑和外周血单个核细胞中特异性表达降低的miRNAs的预测靶基因是神经和造血系统胚胎发育所必需的。我们提出了一个全球性的观点，组织分布的miRNAs与他们的染色体位置和基因组结构。我们还描述了来自miRNAs的顺式调控元件和预测的靶基因的证据，以支持它们的组织特异性功能作用，以调节它们表达的正常组织的生理学。
Measuring the quantity of miRNAs in tissues of different physiological and pathological conditions is an important first step to investigate the functions of miRNAs. Matched samples from normal state can provide essential baseline references to analyze the variation of miRNA abundance. We provided expression data of 345 miRNAs in 40 normal human tissues, which identified universally expressed miRNAs, and several groups of miRNAs expressed exclusively or preferentially in certain tissue types. Many miRNAs with co-regulated expression patterns are located within the same genomic clusters, and candidate transcriptional factors that control the pattern of their expression may be identified by a comparative genomic strategy. Hierarchical clustering of normal tissues by their miRNA expression profiles basically followed the structure, anatomical locations, and physiological functions of the organs, suggesting that functions of a miRNA could be appreciated by linking to the biologies of the tissues in which it is uniquely expressed. Many predicted target genes of miRNAs that had specific reduced expression in brain and peripheral blood mononuclear cells are required for embryonic development of the nervous and hematopoietic systems based on database search. We presented a global view of tissue distribution of miRNAs in relation to their chromosomal locations and genomic structures. We also described evidence from the cis-regulatory elements and the predicted target genes of miRNAs to support their tissue-specific functional roles to regulate the physiologies of the normal tissues in which they are expressed.