Effects of voluntary exercise on synaptic plasticity and gene expression in the dentate gyrus of adult male Sprague-Dawley rats in vivo
Effects of voluntary exercise on synaptic plasticity and gene expression in the dentate gyrus of adult male Sprague-Dawley rats in vivo
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DOI：
10.1016/j.neuroscience.2003.09.029
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发表时间：
2004-01-01
期刊：
影响因子：
3.3
通讯作者：
Christie, BR
中科院分区：
文献类型：
--
作者：
Farmer, J;Zhao, X;Christie, BR
摘要：我们先前已经证明，自愿运动在体外小鼠齿状回（DG）中产生增强的神经发生和长时程增强（LTP）。在本实验中，我们表明，大鼠获得一个运行轮（跑步者）表现出显着更多的短期增强和LTP与θ模式的条件刺激在体内比年龄匹配的同窝（对照）。LTP的增加似乎反映了伴随自愿运动的突触可塑性诱导阈值的改变。弱θ-模式刺激，这并没有产生LTP在对照组，产生了一个强大的和持久的LTP在跑步者。两组LTP的诱导均依赖于N-甲基-D-天冬氨酸（NMDA）受体的激活，并可被竞争性拮抗剂[+/-]-3-[2-羧基哌嗪-4-基]丙烷膦酸阻断。与这些研究结果一致，我们发现NR 2B亚型NMDA受体的mRNA水平在DG的跑步者中特异性增加。除了NR 2B mRNA水平的变化，定量聚合酶链反应分析显示，脑源性神经营养因子（BDNF）和谷氨酸受体5的mRNA水平也显着升高的DG的跑步者，但不是在海马的其他地区。因此，BDNF和特定谷氨酸受体亚型表达的改变可能是运动增强DG神经发生和降低LTP阈值的基础。(C)2004年IBRO。由爱思唯尔有限公司出版。保留所有权利。
'Abstract-We have previously shown that voluntary exercise produces enhanced neurogenesis and long-term potentiation (LTP) in the dentate gyrus (DG) of mice in vitro. In the present experiments we show that rats given access to a running wheel (Runners) exhibit significantly more short-term potentiation and LTP with theta-patterned conditioning stimulation in vivo than do age-matched litter mates (Controls). This increase in LTP appears to reflect an alteration in the induction threshold for synaptic plasticity that accompanies voluntary exercise. Weak theta-patterned stimulation, which did not produce LTP in control subjects, produced a robust and long-lasting LTP in Runners. LTP induction in both groups was dependent upon the activation of N-methyl-D-aspartate (NMDA) receptors, and could be blocked by the competitive antagonist [+/-]-3-[2-carboxypiperazin-4-yl] propanephosphonic acid. Consistent with these findings, we found that mRNA levels for NR2B subtype of NMDA receptor were increased specifically in the DG of Runners. In addition to changes in NR2B mRNA levels, quantitative polymerase chain reaction analysis revealed that brain-derived neurotrophic factor (BDNF) and glutamate receptor 5 mRNA levels were also significantly elevated in the DG of Runners, but not in other areas of the hippocampus. Thus, alterations in the expression of BDNF, and specific glutamate receptor subtypes, may underlie the ability of exercise to enhance neurogenesis and reduce the threshold for LTP in the DG. (C) 2004 IBRO. Published by Elsevier Ltd. All rights reserved.