NOVEL MICRODIALYSIS METHOD TO ASSESS NEUROPEPTIDES AND LARGE MOLECULES IN FREE-MOVING MOUSE
NOVEL MICRODIALYSIS METHOD TO ASSESS NEUROPEPTIDES AND LARGE MOLECULES IN FREE-MOVING MOUSE
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DOI：
10.1016/j.neuroscience.2011.04.035
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发表时间：
2011-06-14
期刊：
影响因子：
3.3
通讯作者：
Morishita, R.
中科院分区：
文献类型：
--
作者：
Takeda, S.;Sato, N.;Morishita, R.
微透析是一种强大的体内技术，用于细胞外液空间内小分子的连续采样。然而，收集更大分子的努力几乎没有成功。在自由活动的动物中鉴定具有生物活性的大分子将是非常有益的。为此，我们已经开发了一种新的微透析方法，允许在清醒的，自由移动的小鼠脑间隙的大分子的一致恢复。使用一种新的“通风”探头与推拉灌注系统，本研究成功地证明了在体内采样的病理生理学重要的大分子在自由移动的小鼠大脑。这一取样系统可监测其浓度的动态变化。总的来说，这种新型的微透析系统将提供机会，以确定在各种生理和病理过程中的病理生理学上重要的蛋白质的表达模式，以更好地了解各种疾病。(C)2011年IBRO。由爱思唯尔有限公司出版。保留所有权利。
Microdialysis is a powerful in vivo technique for the continuous sampling of small molecules within the extracellular fluid space. However, efforts to collect larger molecules have met with little success. To identify biologically active larger molecules in free-moving animals would be of great benefit. For this purpose, we have developed a novel microdialysis method that allows consistent recovery of large molecules from the brain interstitial space in the awake, free-moving mouse. Using a new "vent" probe with a push-pull perfusion system, the present study successfully demonstrated in vivo sampling of pathophysiologically important macromolecules in free-moving mouse brain. This sampling system allowed monitoring of the dynamic changes in their concentrations. Overall, this novel microdialysis system would provide the opportunity to identify the expression patterns of pathophysiologically important proteins in a variety of physiological and pathological processes for a better understanding of various diseases. (C) 2011 IBRO. Published by Elsevier Ltd. All rights reserved.