THE DNAA PROTEIN COMPLEX WITH THE ESCHERICHIA-COLI CHROMOSOMAL REPLICATION ORIGIN (ORIC) AND OTHER DNA SITES
THE DNAA PROTEIN COMPLEX WITH THE ESCHERICHIA-COLI CHROMOSOMAL REPLICATION ORIGIN (ORIC) AND OTHER DNA SITES
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DOI：
10.1016/0092-8674(84)90284-8
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发表时间：
1984-01-01
期刊：
影响因子：
64.5
通讯作者：
KORNBERG, A
中科院分区：
文献类型：
--
作者：
FULLER, RS;FUNNELL, BE;KORNBERG, A
dnaA蛋白与E.大肠杆菌复制起点（oriC）对于染色体复制的起始是必不可少的。基于质粒DNA片段的结合，dnaA蛋白除了识别oriC之外，还识别pSC 101、pBR 322和ColE 1的复制起点内或附近的多个特异性位点; dnaA和X蛋白基因的调节区内;以及IRL-Tn 5中。所有强结合位点共享共同的9 bp [碱基对]序列。图形 **。在oriC内的4个保守位置重复。如DNase I足迹所示，dnaA蛋白结合是高度合作的，覆盖oriC处的250个碱基对和单个9个碱基对序列位点处的100个碱基对。与过滤器结合和核酸酶保护研究一致，通过EM在oriC和其他位点观察到20-30个dnaA单体的复合物。本文还讨论了这些位点的dnaA复合物和9 bp序列的功能。
Specific binding of dnaA protein to the E. coli origin of replication (oriC) is essential for initiation of chromosomal replication. Based on binding of plasmid DNA fragments, dnaA protein recognizes in addition to oriC a number of specific sites within or near the replication origins of pSC101, pBR322 and ColE1; within the regulatory regions of the dnaA and X-protein genes; and in IRL-Tn5. All strong binding sites share a common 9 bp [base pair] sequence, .**GRAPHIC**. repeated at 4 conserved positions within oriC. As shown by DNase I footprinting, dnaA protein binding is highly cooperative, covering 250 bp at oriC and 100 bp at single 9 bp sequence sites. Consistent with filter-binding and nuclease-protection studies, complexes of 20-30 dnaA monomers are visualized at oriC and other sites by EM. The functions of dnaA complexes and 9 bp sequences at these sites are discussed.