Involvement of the Na+/Ca2+ exchanger in ouabain-induced inotropy and arrhythmogenesis in guinea-pig myocardium as revealed by SEA0400
Involvement of the Na+/Ca2+ exchanger in ouabain-induced inotropy and arrhythmogenesis in guinea-pig myocardium as revealed by SEA0400
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DOI：
10.1254/jphs.fp0060911
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发表时间：
2007-02-01
影响因子：
3.5
通讯作者：
Shigenobu, Koki
中科院分区：
文献类型：
--
作者：
Tanaka, Hikaru;Shimada, Hideaki;Shigenobu, Koki
Involvement of the Na+/Ca2+ exchanger in ouabain-induced inotropy and arrhythmogenesis was examined with a specific inhibitor, SEA0400. In right ventricular papillary muscle isolated from guinea-pig ventricle, 1 mu M SEA0400, which specifically inhibits the Na+/Ca2+ exchanger by 80%, reduced the ouabain (1 mu M)-induced positive inotropy by 40%, but had no effect on the inotropy induced by 100 mu M isobutyl methylxantine. SEA0400 significantly inhibited the contracture induced by low Na+ solution. In HEK293 cells expressing the Na+/Ca2+ exchanger, 1 mu M ouabain induced an increase in intracellular Ca2+, which was inhibited by SEA0400. The arrhythinic contractions induced by 3 mu M ouabain were significantly reduced by SEA0400. These results provide pharmacological evidence that the Na+/Ca2+ exchanger is involved in ouabain-induced inotropy and arrhythmogenesis.
