STUDIES OF GENETIC TRANSMISSION OF MAMMARY-TUMOR VIRUS BY C3HF MICE
STUDIES OF GENETIC TRANSMISSION OF MAMMARY-TUMOR VIRUS BY C3HF MICE
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DOI：
10.1002/ijc.2910160606
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发表时间：
1975-01-01
影响因子：
6.4
通讯作者：
VERSTRAETEN, AA
中科院分区：
文献类型：
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作者：
VANNIE, R;VERSTRAETEN, AA
采用放射免疫分析法（RIA）检测C3Hf小鼠、（♀BALB/c ×♂C3Hf）Fl小鼠和（♀C3Hf ×♂BALB/c）Fl小鼠的乳腺肿瘤病毒（MTV）抗原；BALB/c小鼠乳样均为阴性。在分离回交群体中，169只小鼠中有93只（55%）的乳汁中检测到♀BALB/c ×♂（♀BALB/c ×♀C3Hf）病毒抗原。在Bc II型人群（Bc I型母亲的女儿和BALB/c型父亲的女儿）中，两组被区分开来。在第一组中，来自Bc I阳性母亲的110只小鼠中有55只（50%）在乳汁中检测到病毒抗原。在第二组中，阴性母鼠的后代，47只小鼠中有1只呈阳性。这些数据与C3Hf小鼠乳汁中存在病毒抗原的一个显性基因的假设是一致的。该基因（Mtv‐1）似乎与位于7号染色体上的白化位点有关；重组率约为29%。在对Bc - 1小鼠进行的第一次实验中，发现乳汁中含有病毒抗原的小鼠和乳汁中含有病毒抗原的小鼠的肿瘤年龄之间存在显著差异：所有乳汁中含有病毒抗原的小鼠（18只）在7至18个月大的时候患上了乳腺肿瘤，而16只乳汁中含有病毒抗原的小鼠中只有7只在21个月大之前发现了乳腺肿瘤。两亚组小鼠肿瘤中均可检测到病毒抗原（RIA）；然而，在用病毒阴性乳汁喂养的小鼠的肿瘤中，其数量（mU/mg肿瘤）明显较低。虽然C3Hf母鼠的MTV - L可以通过寄养喂养传播给BALB/c小鼠，但在第三哺乳期前收集的乳汁样本中无法检测到病毒抗原；因此，对染色体外传播MTV - L数据的影响不大。
By radioimmunoassay (RIA) mammary tumour virus (MTV) antigens were detected in individual milk samples of C3Hf mice, (♀BALB/c × ♂ C3Hf)Fl mice and (♀C3Hf × ♂ BALB/c)Fl mice; milk samples of BALB/c mice were negative. In the segregating backcross I population, ♀ BALB/c × ♂ (♀BALB/c × ♀ C3Hf) viral antigens were found in the milk of 93 out of 169 mice (55%). In the Bc II population (daughters of Bc I mothers and BALB/c fathers) two groups were distinguished. In the first group, derived from positive Bc I mothers, 55 out of 110 mice (50%) had detectable levels of viral antigens in the milk. In the second group, progeny of negative Bc I mothers, 1 mouse out of 47 was positive. These data are consistent with the assumption that one dominant gene is responsible for the presence of viral antigens in the milk of C3Hf mice. This gene (Mtv‐1) seems to be linked with the albino locus situated on chromosome 7; the recombination percentage was about 29. In the first experiment with Bc I mice a significant difference was found between the tumour ages of the mice with virus‐positive milk and of the mice with virus‐negative milk: all mice (18) with viral antigens in the milk developed mammary tumours at an age ranging from 7 to 18 months, whereas in only 7 out of 16 mice with virus‐negative milk were mammary tumours found before the age of 21 months. Viral antigens were detectable (by RIA) in the tumours of mice of both subgroups; however, the amounts (mU/mg tumour) were significantly lower in the tumours derived from mice with virus‐negative milk. Although MTV‐L of C3Hf mothers could be transmitted to BALB/c mice by foster‐nursing, viral antigens could not be detected in milk samples collected prior to the third lactation period; thus an influence on the data of extrachromosomally transmitted MTV‐L is unlikely.