The expression of natriuretic peptide receptors in developing zebrafish embryos
The expression of natriuretic peptide receptors in developing zebrafish embryos
复制标题
发育中的斑马鱼胚胎中钠尿肽受体的表达。
DOI：
10.1016/j.gep.2018.07.001
复制
发表时间：
2018-09-01
影响因子：
1.2
通讯作者：
Liu, Dong
中科院分区：
文献类型：
--
作者：
Gong, Jie;Chai, Linlin;Liu, Dong
钠尿肽(NP)特异性细胞表面受体有三种亚型：NP受体A(NPRA)、受体B(NPRB)和受体C(NPRC)。它们也分别称为NPR1、NPR2和NPR3。NPS及其受体参与多种细胞和生理过程，包括肾脏、心血管、神经和免疫学方面。然而，到目前为止，对这些受体在非哺乳动物脊椎动物中的表达缺乏系统的分析。在本研究中，在斑马鱼中鉴定了npr1基因的两个版本(npr1a和nprlb)。多序列比对分析表明，斑马鱼NPR与其他物种的NPR有很高的同源性，并具有典型的NPR的特征结构域。整体原位杂交和逆转录聚合酶链式反应分析结果表明，胚胎时期npr1a主要在顶盖脑室、脑、心脏和视网膜表达，而nprlb广泛存在于前肾管。与nprl不同，npr2在胚胎发育过程中主要表达于颧弓和神经管。在72 hpf的斑马鱼胚胎中，npr3在前肾管和锡小体中有表达。在成人中，我们证明了这三种NP受体在脑和肾脏中都高度存在。总之，这些发现将为NPs及其受体在胚胎发育过程中的功能分析提供重要的基础。
There are three isoforms of natriuretic peptide (NP) specific cell surface receptor: NP receptor-A (NPRA), receptor-B (NPRB), and receptor-C (NPRC). They are also known as NPR1, NPR2 and NPR3, respectively. NPs and their receptors were revealed to involve in diverse cellular and physiological processes including renal, cardiovascular, neuronal, and immunological aspects. However, the systematic analysis of the expression of these receptors in non-mammalian vertebrates is thus far lacking. In this study, two versions of the nprl gene (nprla and nprlb) in zebrafish was identified. Multiple sequences alignment analysis showed that zebrafish NPRs shared high homologies with NPRs of other species and possessed a typical signature domain of NPRs. The results of whole mount in situ hybridization and reverse transcription polymerase chain reaction analysis revealed that at embryonic stages, nprla was mainly expressed in tectal ventricle, brian, heart and retina, whereas nprlb was broadly present in anterior pronephric duct. Unlike nprl, npr2 mainly expressed in branchial arches and neural tube during embryonic development. However, npr3 was expressed in pronephric ducts and corpuscle of stannius in zebrafish embryos at 72 hpf. In adults, we demonstrated that all the three NP receptors were highly existed in brain and kidney. Overall, these findings will provide an important basis for the functional analysis of NPs and its receptor during embryonic development.