Acute Electrical Stimulation of Nucleus Ambiguus Enhances Immune Function in Rats
Acute Electrical Stimulation of Nucleus Ambiguus Enhances Immune Function in Rats
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DOI：
10.1017/s0317167100009094
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发表时间：
2008-09
期刊：
影响因子：
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通讯作者：
Ying-Wu Mei;Zhanqing Yang;Wei Wang;De-Guang Song;Xuming Deng;Juxiong Liu
中科院分区：
文献类型：
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作者：
Ying-Wu Mei;Zhanqing Yang;Wei Wang;De-Guang Song;Xuming Deng;Juxiong Liu
背景：到目前为止，许多“免疫活性”的大脑区域已经被识别出来，例如下丘脑核团、大脑奖赏系统；但模糊核（Amb），即延髓神经迷走神经核，在神经免疫调节方面的研究较少。方法：为了对Amb有更深刻的理解和更多的认识，我们研究了Amb的急性电刺激对大鼠胸腺和脾脏活动的影响。使用刺激器以每1s 100μAx5ms x100 Hz 的参数刺激麻醉大鼠的Amb，持续1 分钟。电刺激后0.5、1、2、3小时测定胸腺TGF-β、胸腺素-β4 mRNA水平、体外脾细胞IL-2、IL-6释放量以及脾淋巴细胞增殖情况。结果：结果显示，与对照组相比，在有效的 Amb 刺激后 0.5、1 和 2 小时，伴刀豆球蛋白 A (Con A) 诱导的脾淋巴细胞增殖以及 IL-2 和 IL-6 的释放均显着增强。然而，与对照组相比，在有效Amb刺激后0.5、1和2小时，胸腺中TGF-β和胸腺素-β4 mRNA的水平均显着降低。结论：这些发现表明 Amb 参与动物免疫功能的调节。
Background: Up to now, many “immunoactive” brain areas have been identified, such a hypothalamic nuclei, brain reward system; but the nucleus ambiguous (Amb), a nucleus nervi vagis of medulla oblongata, was less well studied in neuroimmunomodulation. Methods: In order to obtain more profound comprehension and more knowledge on Amb, we studied the effect of acute electrical stimulation of Amb on thymus and spleen activity in rat. A stimulator was applied to stimulate the Amb of the anaesthetic rats using the parameter at 100μAx5ms x100 Hz every 1s for 1 min. The levels of TGF-β and thymosin-β4 mRNA in thymus, the release of IL-2 and IL-6 at splenocyte in vitro and splenic lymphocyte proliferation were measured at hour 0.5,1,2,3 following the electrical stimulation. Results: The results showed that concanavalin A (Con A)-induced splenic lymphocyte proliferation and the release of IL-2 and IL-6 were all significantly enhanced at 0.5, 1, and 2 h following effective Amb stimulation as compared to in the control group. However, as compared to in the control group, the levels of TGF-β and thymosin-β4 mRNA in the thymus were both remarkably reduced at 0.5, 1, and 2 h following effective Amb stimulation. Conclusions: These findings reveal that the Amb participates in the modulation of animal immune functions.