Urinary angiotensinogen as a potential biomarker of severity of chronic kidney diseases
Urinary angiotensinogen as a potential biomarker of severity of chronic kidney diseases
复制标题
DOI：
10.1016/j.jash.2008.04.008
复制
发表时间：
2008-09-01
影响因子：
--
通讯作者：
Yamamoto, Tatsuo
中科院分区：
文献类型：
--
作者：
Kobori, Hiroyuki;Ohashi, Naro;Yamamoto, Tatsuo
我们之前报道过血管紧张素原（AGT）的尿排泄率提供了血管紧张素h依赖性高血压大鼠肾内肾素-血管紧张素系统活性的特定指标。同时，我们最近开发了直接酶联免疫吸附测定法（elisa）来测量人类血浆和尿液中的AGT。本研究旨在验证一种假设，即慢性肾脏疾病（CKD）患者尿中AGT水平升高，并与一些临床参数有关。80名CKD患者（37名女性和43名男性，年龄从18岁到94岁）和7名健康志愿者（2名女性和5名男性，年龄从27岁到43岁）被纳入研究。血浆AGT水平呈正态分布；然而，尿agt -肌酐比值（UAGT/UCre）偏离正态分布。当执行对数变换时，Log（UAGT/UCre）水平呈现正态分布。因此，日志（UAGT/UCre）级别被用于进一步分析。Log（UAGT/UCre）水平与年龄、性别、身高、体重、体重指数、收缩压、舒张压、血清钠水平、血清钾水平、尿钠-肌酐比、血浆肾素活性或血浆AGT水平无关。然而，Log（UAGT/UCre）水平与尿白蛋白-肌酐比、钠排泄分数、尿蛋白-肌酐比和血清肌酐呈显著正相关，与肾小球滤过率呈负相关。与对照组相比，CKD患者的Log（UAGT/UCre）水平显著升高（1.8801 +/- 0.0885 vs 0.9417 +/- 0.1048; P = 0.0024）。这些数据证实了我们之前的报道，并表明一种新的酶联免疫吸附试验是一种有效的方法来测量尿AGT。[J]；2(5): 349 - 354。(C) 2008年美国高血压学会。版权所有。
We previously reported that urinary excretion rates of angiotensinogen (AGT) provide a specific index of the activity of the intrarenal renin-angiotensin system in angiotensin H-dependent hypertensive rats. Meanwhile, we have recently developed direct enzyme-linked immunosorbent assays (ELISAs) to measure plasma and urinary AGT in humans. This study was performed to test a hypothesis that urinary AGT levels are enhanced in chronic kidney disease (CKD) patients and con-elated with some clinical parameters. Eighty patients with CKD (37 women and 43 men, from 18 to 94 years old) and seven healthy volunteers (two women and five men, from 27 to 43 years old) were included. Plasma AGT levels showed a normal distribution; however, urinary AGT-creatinine ratios (UAGT/UCre) deviated from the normal distribution. When a logarithmic transformation was executed, Log(UAGT/UCre) levels showed a normal distribution. Therefore, Log(UAGT/UCre) levels were used for further analyses. Log(UAGT/UCre) levels were not con-elated with age, gender, height, body weight, body mass index, systolic blood pressure, diastolic blood pressure, serum sodium levels, serum potassium levels, urinary sodium-creatinine ratios, plasma renin activity, or plasma AGT levels. However, Log(UAGT/UCre) levels were significantly con-elated positively with urinary albumin-creatinine ratios, fractional excretion of sodium, urinary protein-creatinine ratios, and serum creatinine, and correlated negatively with estimated glomerular filtration rate. Log(UAGT/UCre) levels were significantly increased in CKD patients compared with control subjects (1.8801 +/- 0.0885 vs. 0.9417 +/- 0.1048; P = .0024). These data confirmed our earlier report and showed that a new ELISA assay is a valid approach for measuring urinary AGT. J Am Soc Hypertens 2008;2(5): 349-354. (C) 2008 American Society of Hypertension. All rights reserved.