Analysis of Lhc family genes reveals development regulation and diurnal fluctuation expression patterns in Cyperus esculentus, a Cyperaceae plant
Analysis of Lhc family genes reveals development regulation and diurnal fluctuation expression patterns in Cyperus esculentus, a Cyperaceae plant
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DOI：
10.1007/s00425-023-04092-5
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发表时间：
2023-02
期刊：
影响因子：
4.3
通讯作者：
Z. Zou;Yanhua Xiao;Li Zhang;Yongguo Zhao
中科院分区：
文献类型：
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作者：
Z. Zou;Yanhua Xiao;Li Zhang;Yongguo Zhao
Main conclusionSixteenLhcgenes representing 13 phylogenetic groups were identified from the full-length transcriptome of tigernut, exhibiting development regulation and diurnal fluctuation expression patterns in leaves.AbstractNuclear encoded light-harvesting chlorophylla/b-binding (Lhc) proteins play indispensable roles in oxygenic photosynthesis. In this study, we present the first transcriptome-based characterization ofLhcfamily genes in tigernut (Cyperus esculentusL.), a Cyperaceae C4plant producing oil in underground tubers. A number of 16Lhcgenes representing 13 phylogenetic groups identified from the full-length tigernut transcriptome are equal to that found in bothCarex littledalei(another Cyperaceae plant) and papaya, slightly more than 15 members present in both rice and jatropha, but relatively less than 18, 20, and 21 members present in sorghum, cassava, and Arabidopsis, respectively. Nevertheless, nearly one-vs-one orthologous relationship was observed in most groups, though some of them are no longer located in syntenic blocks and species-specific expansion was frequently found in Lhcb1. Comparative genomics analysis revealed that the loss of two groups (i.e., Lhca2 and Lhca5) inC. littledaleiis species-specific, sometime after the split with tigernut, and the expansion of Lhcb1 was mainly contributed by tandem duplication as observed in most species. Interestingly, a transposed duplication, which appears to be shared by monocots, was also identified in Lhcb1. Further transcriptome profiling revealed a predominant expression pattern of mostCeLhcfamily genes in photosynthetic tissues and enhanced transcription during leaf maturation, reflecting their key roles in light absorption. Moreover, qRT-PCR analysis revealed an apparent diurnal fluctuation expression pattern of 11 dominantCeLhcgenes. These findings not only highlight species-specific evolution ofLhcgenes in the Cyperaceae family as well as the monocot lineage, but also provide valuable information for further functional analysis and genetic improvement in tigernut.
