TGF-beta1 attenuated branching morphogenesis of embryonic murine submandibular gland through Smad3 activation
TGF-beta1 attenuated branching morphogenesis of embryonic murine submandibular gland through Smad3 activation
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TGF-β1通过Smad3激活减弱胚胎小鼠颌下腺的分支形态发生
DOI：
10.1111/ahe.12295
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Li C. J.
中科院分区：
文献类型：
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作者：
Gao P.;Qiao X. H.;Gou L. M.;Huang Y.;Li Q. H.;Li L. J.;Wang X. Y.;Li C. J.
转化生长因子-β1（Transforming growth factor-β 1，TGF-β1）在多种生理和病理过程中起着重要的调节作用。本研究旨在探讨TGF-β1在涎腺分支形态发生中的作用。我们从小鼠胚胎中收获并培养下颌下唾液腺（SMG），然后用外源性TGF-β1或其中和抗体、Smad 3抑制剂或Smad 3小干扰RNA（siRNA）处理。我们的结果表明，TGF-β1通过激活Smad 3减弱胚胎小鼠SMG的分支形态发生，从而对唾液腺发育发挥负调节作用。
Transforming growth factor‐β1 (TGF‐β1) plays several crucial regulatory roles in multiple physiological and pathological processes. The aim of this work was to investigate the role of TGF‐β1 in branching morphogenesis of salivary gland. We harvested and cultured submandibular salivary glands (SMGs) from murine embryos, which were then treated with exogenous TGF‐β1, or its neutralized antibody, Smad3 inhibitor, or Smad3 small interfering RNA (siRNA). Our results suggested that TGF‐β1 attenuated branching morphogenesis of embryonic murine SMG via Smad3 activation, thus playing a negative regulatory role in salivary gland development.