Significance of eukaryotic translation elongation factor 1A in tobacco mosaic virus infection
Significance of eukaryotic translation elongation factor 1A in tobacco mosaic virus infection
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DOI：
10.1007/s00705-009-0571-x
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发表时间：
2010-02
影响因子：
2.7
通讯作者：
Y. Yamaji;K. Sakurai;K. Hamada;K. Komatsu;J. Ozeki;A. Yoshida;A. Yoshii;T. Shimizu;S. Namba;T. Hibi
中科院分区：
文献类型：
--
作者：
Y. Yamaji;K. Sakurai;K. Hamada;K. Komatsu;J. Ozeki;A. Yoshida;A. Yoshii;T. Shimizu;S. Namba;T. Hibi
真核翻译延伸因子1A （eEF1A）已被证明与病毒RNA依赖的RNA聚合酶和烟草花叶病毒（TMV）的3 '端基因组RNA相互作用。在这项研究中，我们证明了利用马铃薯病毒X载体通过病毒诱导的基因沉默来下调feef1amrna水平，可以显著减少TMV RNA的积累和TMV感染的传播。ef1a沉默的烟叶的翻译活性没有受到严重影响。总之，这些结果表明eEF1A在TMV感染中起重要作用。
Eukaryotic translation elongation factor 1A (eEF1A) has been shown to interact with both the viral RNA-dependent RNA polymerase and the 3′-terminal genomic RNA of tobacco mosaic virus (TMV). In this study, we demonstrated that the down-regulation ofeEF1AmRNA levels by virus-induced gene silencing using potato virus X vector dramatically reduced the accumulation of TMV RNA and the spread of TMV infection. The translation activity of theeEF1A-silencedNicotiana benthamianaleaves was not severely affected. Collectively, these results suggest an essential role of eEF1A in TMV infection.