Oriented immobilization of antibodies by a self-assembled monolayer of 2-(biotinamido)ethanethiol for immunoarray preparation
Oriented immobilization of antibodies by a self-assembled monolayer of 2-(biotinamido)ethanethiol for immunoarray preparation
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DOI：
10.1016/j.colsurfb.2005.07.007
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发表时间：
2006-01-15
影响因子：
5.8
通讯作者：
Ha, KS
中科院分区：
文献类型：
--
作者：
Jung, SH;Son, HY;Ha, KS
抗体的定向在制备免疫阵列中是非常重要的，以保持抗体在固体表面的活性。因此，我们合成了一种新的双功能化合物，2-（生物胺）乙硫醇，并利用光谱SPR生物传感器研究了该硫醇化合物在免疫阵列上分析抗体-抗原相互作用是否有用。用核磁共振波谱和质谱对合成的有机硫醇进行了表征。2-（生物胺）乙硫醇在金表面形成单层，并通过链亲和素和生物素化蛋白g适当地固定抗体。2-（生物胺）乙硫醇和巯基己醇的最佳摩尔比为1:2。因此，2-（生物胺）乙硫醇是一种有用的双功能连接剂，用于在金表面制备免疫阵列。(c) 2005 Elsevier B.V.版权所有
Orientation of antibodies is very important in the preparation of immunoarrays to keep the activity of antibodies on solid surfaces. Thus, we synthesized a new bifunctional compound, 2-(biotinamido)ethanethiol, and investigated whether the thiol compound is useful to analyze antibody-antigen interactions on immunoarrays with a spectral SPR biosensor. The synthesized organic thiol was characterized by nuclear magnetic resonance spectroscopy and mass spectrometry. 2-(Biotinamido)ethanethiol formed a monolayer on a gold surface and properly immobilized antibodies via streptavidin and biotinylated protein G. Optimal molar ratio of 2-(biotinamido)ethanethiol and mercaptohexanol for antigen-antibody interactions was 1:2. Thus, 2-(biotinamido)ethanethiol is an useful bifunctional linker in the preparation of immunoarrays on gold surfaces. (c) 2005 Elsevier B.V. All rights reserved.