Glass-like protein condensate for the long-term storage of proteins
Glass-like protein condensate for the long-term storage of proteins
复制标题
用于长期储存蛋白质的玻璃状蛋白质浓缩物
DOI：
10.1016/j.ijbiomac.2021.04.017
复制
发表时间：
2021
影响因子：
8.2
通讯作者：
Shiraki Kentaro
中科院分区：
文献类型：
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作者：
Nakauchi Yoshitaka;Nishinami Suguru;Shiraki Kentaro
在制药和生物技术中的应用需要蛋白质在环境温度下的长期储存。冻干是在环境温度下稳定蛋白质的通用方法，尽管其冷冻和干燥过程对蛋白质结构产生负面影响。在这项研究中，我们展示了一种玻璃状蛋白质缩合物（GLPC）作为一种新的方法，在环境温度下的蛋白质稳定。各种类型的蛋白质，包括免疫球蛋白G，γ球蛋白，白蛋白和糜蛋白酶，在超离心和自然干燥过程中形成玻璃态，而倾向于结晶的蛋白质，如鸡蛋清溶菌酶，没有。GLPC的特征在于透明的固态，类似于干燥的玻璃球。重要的是，GLPC在生理浓度下容易溶解在盐水溶液中，从而保留其天然结构和功能。即使在环境温度下孵育1年后，GLPC仍保持其天然结构。这些结果提供了一个框架，在环境温度下的蛋白质保存方法，而不是冻干的发展。
Long-term storage of proteins at ambient temperature is required for applications in pharmaceutics and biotechnology. Lyophilization is a versatile approach for stabilizing proteins at ambient temperature, although its freezing and drying processes negatively affect the protein structure. In this study, we show a glass-like protein condensate (GLPC) as a new method for protein stabilization at ambient temperature. Various protein types, including immunoglobulin G, gamma globulin, albumin, and chymotrypsin, formed a glassy state during ultracentrifugation and natural drying, while proteins that tend to crystalize, such as hen egg-white lysozyme, did not. The GLPCs were characterized by a transparent solid state, similar to a dry glass ball. Importantly, the GLPCs were dissolved easily in saline solution at a physiological concentration, thereby retaining their native structures and functions. The GLPCs preserved their native structures even after 1 year of incubation at ambient temperature. These results provide a framework for the development of protein preservation methods at ambient temperature other than lyophilization.