A soluble ecto-ATPase from Tetrahymena thermophila: purification and similarity to the membrane-bound ecto-ATPase of smooth muscle.
A soluble ecto-ATPase from Tetrahymena thermophila: purification and similarity to the membrane-bound ecto-ATPase of smooth muscle.
复制标题
来自嗜热四膜虫的可溶性外源 ATP 酶：纯化及其与平滑肌膜结合外源 ATP 酶的相似性。
DOI：
10.1006/abbi.1996.9779
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发表时间：
1997
影响因子：
3.9
通讯作者：
Hennessey,TM
中科院分区：
文献类型：
--
作者：
SmithJr,TM;Kirley,TL;Hennessey,TM
嘌呤能激动剂，如三磷酸腺苷，被认为是快速营养和约69,000Da大小的兴奋性神经递质在中枢和外周的天然形式的层析。外周神经系统(2)和变形的可溶性酶的细胞间调节剂显示出一般特征--许多其他细胞功能。专一的E-型胞外ATPase的特性，如钙/Rine能受体，一般的重要性体现在核苷或镁能受体的参与，ATP和其他核三磷酸对心脏、血管和平滑肌的水解性作用，激动型三磷酸盐(但不包括对神经元的核苷二磷酸和抑制作用，以及对离子、磷酸的作用)，以及对常见的ATPase抑制激素、外分泌腺、血小板、肥大细胞和炎症因子(钒、叠氮、哇巴因、N-乙基马来酰亚胺和托里细胞分泌)的不敏感性[3]。对氯汞苯磺酸盐)。此外，由于四膜虫可以检测到并对膜结合的细胞外环境做出反应(通过多克隆透明膜试验显示，在免疫学上与其抗体中的GTP纳摩尔浓度相似)，我们一直对
Purinergic agonists, such as ATP, are considered to be fast trophoresis and approximately 69,000 Da by size ex-excitatory neurotransmitters in both the central and periph-clusion chromatography of the native form. The puri-eral nervous systems (2) and intercellular modulators of fied soluble enzyme displays the general characteris-many other cell functions. The general importance of pu-tics of a dedicated E-type ecto-ATPase such as Ca2/rinergic reception is seen in the involvement of nucleoside or Mg2/dependence, hydrolysis of ATP and other nu- triphosphates on cardiac, vascular and smooth muscle, excit-cleoside triphosphates (but not nucleoside diphos- atory and inhibitory effects on neurons, and effects on ion, phates) and insensitivity to common ATPase inhibi- hormone, exocrine gland, platelet, mast cell, and inflamma-tors (vanadate, azide, ouabain, N-ethylmaleimide and tory cell secretions (3). p-chloromercuriphenyl sulfonate). It was further Since Tetrahymena can detect and respond (in a chemore-shown to be immunologically similar (by polyclonal pellent assay) to nanomolar concentrations of GTP in their antibodies) to both the membrane-bound ecto- extracellular environment (4), we have been interested in