HUMAN PAPILLOMAVIRUS DETECTION BY HYBRID CAPTURE AND ITS POSSIBLE CLINICAL USE
HUMAN PAPILLOMAVIRUS DETECTION BY HYBRID CAPTURE AND ITS POSSIBLE CLINICAL USE
复制标题
DOI：
10.1136/jcp.47.7.649
复制
发表时间：
1994-07-01
影响因子：
3.4
通讯作者：
VOUSDEN, KH
中科院分区：
文献类型：
--
作者：
FARTHING, A;MASTERSON, P;VOUSDEN, KH
目的，以确定的敏感性的杂交捕获方法的人乳头瘤病毒（HPV）的检测和潜在的临床用途作为一种筛选方法，用于鉴定宫颈上皮内neoplasia.Methods致癌类型的HPV的存在进行了测试，在阴道镜下从宫颈采取的样本中，并与60例患者的聚合酶链反应（PCR）检测比较。两组结果均与活检和涂片细胞学病理结果进行了校正，结果显示杂交捕获检测与PCR的一致率为86%。83%的CIN3病变，62%的CIN2，59%的CIN1和21%的正常对照组的致癌HPV type.Conclusion杂交捕获检测方法是可靠的，敏感的，易于使用。在细胞学筛查中增加HPV检测将发现更大比例的宫颈不典型增生，假阳性率更高。
Aims-To determine the sensitivity of the hybrid capture method for human papillomavirus (HPV) detection and potential clinical uses as a screening method for the identification of cervical intraepithelial neoplasia.Methods-The presence of oncogenic types of HPV was tested for in samples taken from the cervix at colposcopy, and compared with detection by polymerase chain reaction (PCR) in 60 patients. Both sets of results were corrected with the pathology determined by biopsy and smear cytology.Results-Hybrid capture detection showed 86% agreement with PCR. Eighty three percent of CIN 3 lesions, 62% of CIN 2, 59% of CIN 1 and 21% of normal controls were positive for oncogenic HPV types.Conclusion-The hybrid capture detection method is reliable, sensitive, and easy to use. The addition of HPV testing to cytological screening would detect a greater proportion of cervical dysplasia with a higher false positive rate.