Distribution of Porphyromonas gingivalis fimA genotypes in Japanese children and adolescents
Distribution of Porphyromonas gingivalis fimA genotypes in Japanese children and adolescents
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DOI：
10.1902/jop.2005.76.5.674
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发表时间：
2005-05-01
影响因子：
4.3
通讯作者：
Ooshima, T
中科院分区：
文献类型：
--
作者：
Tamura, K;Nakano, K;Ooshima, T
背景：牙龈卟啉单胞菌被认为是牙周病最重要的病原菌之一，其fimA基因分为6个变异体(I~V和Ib型)。从牙周炎患者中分离出的牙龈假单胞菌以II型为主，其次为IV型，而I型在健康成人中普遍存在。方法：从大阪大学口腔医院就诊的464名3~18岁儿童中采集650份唾液样本。结果：15例(3.23%)Pggivalis阳性患者对FIMA基因检测阳性，其中4例(3.23%)Pggivalis阳性患者对II型FIMA基因检测阳性，4例(1例)FIMA基因阳性，2例FIMA基因阳性。此外，在年龄较大的人群中，有3名受试者检出了IV型。结论：我们的发现表明，少数儿童患有牙周炎，而且II型和IV型FIMA的分布极低。此外，一些青少年被发现具有IV型FIMA基因，该基因可能与成人牙周炎有关，而不是I、III和V型。
Background: Porphyromonas gingivalis is considered to be one of the most important pathogens in periodontal disease and its fimA genes have been classified into six variants (types I through V and Ib). A majority of the P gingivalis organisms isolated from periodontitis patients are type II, followed by type IV, while type I is prevalent in healthy adults.Methods: A total of 650 saliva samples were taken from 464 children (3 to 18 years of age), who visited Osaka University Dental Hospital. Genomic DNA was extracted from each sample and analyzed using a polymerase chain reaction (PCR) method with P gingivalis-specific primers, followed by an additional PCR assay to determine the fimA genotypes for P gingivalis-positive subjects.Results: Fifteen (3.23%) of the subjects were P gingivalis-positive and none of those samples showed a positive reaction to the type II fimA-specific primers, while four, one, and two subjects were shown to be positive for the type I, Ib, and III genotypes, respectively. In addition, the type IV genotype was detected in three subjects in the older age group.Conclusions: Our findings suggest that a limited number of children harbor P gingivalis, and that the distribution of type II and IV fimA genotypes is extremely low. Further, some adolescents were found to possess the type IV fimA genotype which has been shown to be possibly related to adult periodontitis, in contrast to types I, III, and V.