The biomaterial-mediated healing of critical size bone defects in the ovariectomized rat
The biomaterial-mediated healing of critical size bone defects in the ovariectomized rat
复制标题
DOI：
10.1007/s00198-014-2656-y
复制
发表时间：
2014-05-01
影响因子：
4
通讯作者：
Fernandes, M. H.
中科院分区：
文献类型：
--
作者：
Durao, S. F.;Gomes, P. S.;Fernandes, M. H.
本研究证明了在卵巢切除大鼠模型中建立的临界尺寸颅骨缺损中生物材料介导的骨再生受损。成骨细胞基因表达受损，雌激素受体和成脂标记物表达改变，支持组织学和显微断层扫描证据，这项工作的目的是解决骨再生过程中的卵巢切除大鼠模型，通过评估一个颅骨临界尺寸缺损植入生物相容性牛骨矿物质graft.Animals随机分为两组：卵巢切除术（双侧卵巢切除术）和假手术（对照手术）。8周后，对所有动物进行手术双皮质开颅术（5 mm圆形临界尺寸缺损），并用生物相容性矿物移植物填充。在移植物植入后1、3和6个月对动物实施安乐死（n = 10），并通过放射学、显微断层摄影、组织学、组织形态学和基因表达技术对原位骨再生过程的结果进行盲法评价。同样，显微断层扫描和组织学分析的定性评价以及组织形态学指标的定量数据显示，在测定的时间点，Ovx动物的骨再生减少。在3个月和6个月时达到显著差异。基因表达分析显示，在Ovx动物的再生骨中，成骨细胞相关基因的表达减少，雌激素受体和脂肪形成标志物的表达改变。可能需要考虑生物材料介导的骨组织愈合中骨坏死状况的潜在临床意义/再生过程
This study demonstrated an impaired biomaterial-mediated bone regeneration in a critical sized calvarial defect established within an ovariectomized rat model. Histological and microtomographic evidences were supported by an impaired osteoblastic gene expression and altered expression of estrogen receptors and adipogenic markers.This work aims to address the bone regeneration process in the ovariectomized rat model, by assessing a calvarial critical size defect implanted with a biocompatible bovine bone mineral graft.Animals were randomly divided into two groups: Ovx (bilateral ovariectomy) and Sham (control surgery). Following 8 weeks, all animals were submitted to a surgical bicortical craniotomy (5-mm circular critical size defect), which was filled with a biocompatible mineral graft. Animals were euthanized at 1, 3, and 6 months following graft implantation (n = 10), and results on the orthotopic bone regeneration process were blindly evaluated by radiographic, microtomographic, histological, histomorphometric, and gene expression techniques.In the attained model, in both Sham and Ovx groups, the bone regenerative process was found to occur in a slow-paced manner. Likewise, a qualitative evaluation of the microtomographic and histological analysis, as well as quantitative data from histomorphometric indexes, revealed reduced bone regeneration in Ovx animals, at the assayed time points. Significant differences were attained at the 3 and 6 months. Gene expression analysis revealed a reduced expression of osteoblastic-related genes and an altered expression of estrogen receptors and adipogenic markers, within the regenerating bone of Ovx animals.Due to the similarities between the osteoporotic animal model and the human condition of postmenopausal osteoporosis, it might be relevant to consider the potential clinical implication of the osteoporotic condition in the biomaterial-mediated bone tissue healing/regeneration process.